摘要
目的：分析乌司他丁对肺癌患者放疗后急性肺损伤的保护作用及相关分子机制。方法：将2013年12月~2015年12月在本院接受放疗并发生急性肺损伤的78例患者随机分为观察组及对照组,对照组接受对症治疗,观察组接受对症＋乌司他丁治疗,对比两组治疗后的血清生长因子、炎症因子、病情相关蛋白含量以及肺泡灌洗液中P38MAPK信号通路分子的表达量。结果：治疗10d后,观察组血清肝细胞生长因子（HGF）、角细胞生长因子（KGF）、血管内皮生长因子（VEGF）、白介素-1β（IL-1β）、白介素-8（IL-8）、白介素-10（IL-10）、白介素-18（IL-18）、白介素-13（IL-13）、降钙素原（PCT）、S100A8、S100A9、肺表面活性蛋白D（SP-D）含量显著低于对照组（P〈0.05）,Clara细胞蛋白的含量显著高于对照组（P〈0.05）;肺泡灌洗液中磷酸化p38MAPK、丝裂原活化蛋白激酶（MAPK）、MKK3/6、ATF-2的蛋白表达量显著低于对照组（P〈0.05）。结论：乌司他丁可缓解放疗后急性肺损伤患者的整体病情,具体机制与P38MAPK信号通路相关。
Objective： To analyze the protective effect of ulinastatin on acute tung injury after radiotherapy in patients with lung cancer and the related molecular mechanism. Methods： A total of 78 patients who received radiotherapy and developed acute lung injury in our hospital between December 2013 and December 2015 were randomly divided into observation group and control group, control group received symptomatic treatment, observation group received symptomatic -F ulinastatin treat- ment, and the content of growth factors, inflammatory factors, disease-related proteins in serum as well as the expression of P38MAPK signaling pathway molecules in alveolar favage fluid were compared between two groups of patients after treatment. Results： A total of 10 d after treatment, hepatocyte growth factor （HGF） ; keratinocyte growth factor （KGF）, Vascular endo- thelial growth factor （VEGF）, IL-1β, IL-8, IL-10, IL-18, IL-13, procalcitonin （PCT）, S100A8, S100A9 and pulmonary sur- factant-associated protein D （SP-D） content in serum of observation group were significantly lower than those of control group （P〈0.05） while Clara cell protein content was significantly higher than that of control group （P 〈0.05）; phosphorylatedp38MAPK, MAPK, MKK3/6 and ATF-2 protein expression levels in alveolar lavage fluid were significantly lower than those of control group （P〈0.05）. Conclusions： Ulinastatin can alleviate the overall condition in patients with acute lung injury after radiotherapy, and the specific mechanism is associated with P38MAPK signaling pathway.
出处
《海南医学院学报》
CAS
2016年第24期3042-3045,共4页
Journal of Hainan Medical University
基金
湖北省教育厅科学研究计划项目(D20110261)~~
