摘要
为了明确河南省黄花蒿挥发油的成分和抑菌活性,采用水蒸气蒸馏法提取采自河南信阳的黄花蒿挥发油,利用气相色谱-质谱联用法(GC-MS)进行组分分析,并测定挥发油的熏蒸抑菌作用。结果表明:从黄花蒿挥发油中共分离到28个组分,鉴定出其中24个,占挥发油总量的95.324%,其主要组分为蒿酮(46.654%)、桉油精(13.422%)、樟脑(9.648%)等;挥发油对水稻纹枯病菌、番茄灰霉病菌、小麦赤霉病菌和油菜菌核病均有强烈的熏蒸抑制作用,有效中浓度(EC50)分别为0.78μL/皿、0.89μL/皿、1.32μL/皿和1.52μL/皿。说明河南省黄花蒿挥发油可在农业上作为杀菌剂使用,具有一定的开发价值。
In order to determine the chemical constituents and antifungal activity of essential oil from Artemisia annua Linn,essential oil from A. annua Linn in Xinyang city of Henan province was extracted by steam distillation,and their chemical constituents were analyzed by GC-MS. The antifungal activity of essential oil was also studied. The results showed that 28 ingredients were found,and 24 of them were identified,accounting for 95. 324% of the total essential oil from A. annua Linn. The main chemical constituents were artemisia ketone( 46. 654%),eucalyptol( 13. 422%),camphor( 9. 648%),etc. The essential oil showed strong fumigation antifungal effect on Rhizoctonia solani,Botrytis cinerea,Fusarium graminearum and Sclerotinia sclerotiorum,and the EC50 values were 0. 78 μL / dish,0. 89 μL / dish,1. 32μL /dish and 1. 52 μL /dish,respectively. The essential oil can be used as a fungicide in agriculture and possesses a certain developing value.
出处
《河南农业科学》
CSCD
北大核心
2016年第12期105-109,共5页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金项目(31401798)
河南省科技攻关项目(122102310257)
