摘要
目的:建立HPLC-RID测定天麻蜜环菌片中的D-无水葡萄糖含量的方法。方法:Prevail Carbihyrate ES糖色谱柱(250mm×4.6mm,5μm),柱温为35℃,流动相为乙腈-水(75:25),流速为1.0m L/min。结果:D-无水葡萄糖的回归方程为y=3.4797x+0.0215(r=0.9991)。线性范围为0.6275μg/m L^2.51μg/m L;结论:该法可为控制蜜环菌粉制剂的内在质量提供依据。
Objective： To establish a HPLC-RID method for determination of D-Glucose anhydrous in Gastrodia Tuder Halimasch Tablets; Methods： The analytical column was Prevail Carbihyrate ES（250 mm × 4. 6mm,5μm） with the temperature of 35℃; mobile phase was acetonitrile-water（75： 25）; The flow rate was 1. 0m L/min. Results： The regression equation of Anhydrous Glucose was y= 3. 4797 x ＋ 0. 0215（r = 0. 9991）,linear range was 0. 6275μg/m L - 2. 51μg/m L; Conclusion： The method provides a basis for internal quality control of Armillaria powder preparation。
出处
《江西中医药大学学报》
2016年第6期62-63,共2页
Journal of Jiangxi University of Chinese Medicine
