摘要
目的探讨差异蛋白(TM9SF1,AZU1)在颅内动脉瘤形成和破裂中的作用机制.方法收集26例颅内动脉瘤夹闭手术患者的动脉瘤血管壁组织,术中取同一患者头皮断裂(>2 mm)的颞浅动脉(STA)作对照,采用Western blot和免疫组化法分析TM9SF1,AZU1在STA和颅内动脉瘤血管壁组织中的含量和表达差异.结果 Western blot显示:颅内动脉瘤血管壁组织中TM9SF1和AZU1含量较STA组织分别增加5.5,7.8倍,差异具有统计学意义(P<0.05).免疫组化显示:STA组织中TM9SF1,AZU1呈阴性或弱阳性表达,颅内动脉瘤血管壁组织中TM9SF1和AZU1呈阳性或强阳性表达,动脉瘤血管壁组织中TM9SF1,AZU1染色分级明显高于STA组织,差异具有统计学意义(P<0.05).结论 TM9SF1,AZU1可能参与了颅内动脉瘤发病的分子机制.
Objective To investigate the effect mechanism of differentially expressed proteins （TM9SF1 and AZU1 ） in the wall of intracranial aneurysm on the formation and rupture of aneurysm. Method Aneurysm vascular wall tissues of 26 cases with intracranial aneurysm and undergoing the clipping operation of aneurysm were collected, and the superficial temporal artery （STA） at the same side of the same patient was taken as control through the ruptured scalp during the operation（ 〉2 mm）. The content and expression difference of AZU1 and TM9SF1 in the vascular wall of STA and intraeranial aneurysms were analyzed by blot Western and immunohistochemistry. Results Blot Western showed that the content of TM9SF1 and AZU1 in the vascular wall tissue of intracranial aneurysm increased by 5.5 times and 7.8 times in STA tissue, respectively, with statistically significant differences （P〈0.05）. Immunohistochemistry showed that TM9SF1 and AZU1 in the STA tissue presented a negative or weakly positive expression, but those intracranial aneurysm vessel wall tissue presented a positive or strong positive expression, and the staining grading of TM9SF1 and AZU1 in the aneurysm vascular wall tissue was significantly higher than that in the STA tissue, showing a statistically significant difference （ P〈0.05 ）. Conclusion The continuously high expression of AZU1 and TM9SF1 is involved in the inflammation and aneurysm vessel wall protein degradati in the process of the formation and rupture of intracranial aneurysrns.
出处
《北华大学学报（自然科学版）》
CAS
2016年第6期756-759,共4页
Journal of Beihua University（Natural Science）
基金
辽宁省科学技术研究项目(2014022023)
关键词
差异蛋白
颅内动脉瘤
破裂
机制
differentially expressed protein
intracranial aneurysm
rupture
mechanism
