摘要
目的:研究脉冲射频(pulsed radiofrequency,PRF)大鼠慢性坐骨神经缩窄损伤(chronic constriction injury of sciatic nerve,CCI)模型背根神经节(dorsal root ganglion,DRG)能否抑制脊髓小胶质细胞中干扰素调节因子8(interferon regulatory factor 8,IRF8)表达而减轻疼痛。方法:雄性SD大鼠120只随机均分为4组:假手术组(Sham)、CCI组、CCI+PRF组、CCI+NPRF组,每组30只。采用结扎一侧坐骨神经主干制作大鼠CCI模型。CCI+PRF组于制模后第7 d,对术侧L4-5背根神经节进行脉宽20 ms、脉冲频率2 Hz,42℃,持续6 min的PRF治疗。CCI+NPRF组仅在相同位置放置射频电极,无脉冲治疗,持续6 min。制模前测量各组大鼠基础阈值,制模后1、3、5、7 d,PRF后1、3、5、7、14 d四组大鼠均接受行为学、痛阈测定。制模后7 d,PRF后7、14 d western blot检测IRF8蛋白表达。制模后7 d,PRF后14 d免疫荧光法检测Iba1表达。结果:与假手术组比,CCI组、CCI+PRF组、CCI+NPRF组大鼠于神经损伤后痛阈均显著降低(P<0.05),脊髓IRF8、Iba1表达上调(P<0.05)。脉冲射频后CCI+PRF组与治疗前比较痛阈升高(P<0.05),并显著高于CCI组和CCI+NPRF组(P<0.05),脊髓IRF8、Iba1表达则相应减少。结论:脉冲射频可缓解大鼠CCI模型神经病理性疼痛,其机制可能与抑制大鼠脊髓IRF8表达、小胶质细胞活化有关。
Objective： To investigate whether pulsed radiofrequency（PRF） on dorsal root ganglion（DRG） of rats with chronic constriction injury of sciatic nerve（CCI） could restrain the expression of IRF8 protein in spinal microglia to alleviate neuropathic pain. Methods： 120 adult male Sprague-Dawley rats were randomly assigned into four groups as follows（n=30 for each）： Sham group, CCI group, CCI＋PRF group, and CCI＋NPRF group. All rats except those in Sham group were subjected to chronic constriction injury of sciatic nerve. On the 7th postoperative day in CCI＋PRF group, PRF（2 bursts/s, duration = 20 milliseconds） was applied on the ipsilateral L4-5 DRG for 6 min, with a maximum temperature of 42 ℃; while in CCI＋NPRF group, PRF needles were placed adjacent to the L4-5 DRG for 6 min without radiofrequency current. Basal pain thresholds were measured preparation, and pain behavioral changes were measured before operation, on 1st, 3rd, 5th, 7th postoperative day and 1st, 3rd, 5th, 7th, 14 th after PRF treatment, respectively. The expressions of IRF8 protein were measured in spinal cords by using western blot in each group on 7th postoperative day and 7th, 14 th after PRF treatment. And the expressions of Iba1 were measured in spinal cords by using immunofluorescence in each group on 7th and 14 th postoperative day after PRF treatment, respectively. Results： The pain thresholds at each time points were significantly decreased（P 0.05）, whereas the expressions of IRF8 and Iba1 in spinal cords after nerve transection were significantly increased in CCI group, CCI＋PRF group and CCI＋NPRF group than those in Sham group（P 0.05）. After PRF treatment in CCI＋PRF group, pain thresholds were up-regulated and the expressions of IRF8 and Iba1 in spinal cords were down-regulated significantly than those before PRF treatment, at the same points in CCI group and CCI＋NPRF group（P0.05）. Conclusions： Our results demonstrated that the neuropathic pain induced by CCI was attenuated by PRF pr
作者
方向宇
徐雪汝
刘荣国
米立梅
许云禄
FANG Xiang-Yu XU Xue-Ru LIU Rong-Guo MI Li-Mei XU Yun-Lu(Department of Pain Management, Fujian Provincial Hospital, Provincial Clinic college of Fujian Medical University, Fuzhou, 350001 Fujian Medical University, Fuzhou, 350000, China)
出处
《中国疼痛医学杂志》
CAS
CSCD
2016年第11期816-822,共7页
Chinese Journal of Pain Medicine
基金
福建省医学创新课题(项目号:2016-CX-3)
福建省卫生系统第四批学术技术带头人培养经费
关键词
神经病理性疼痛
脉冲射频
背根神经节
小胶质细胞
IRF8
Neuropathic pain
Pulsed radiofrequency
Dorsal root ganglion
Microglia
Interferon regulatory factor 8
