摘要
阿勒泰羊的抗寒性能与其肥硕的尾脂(臀脂)高度相关。试验旨在通过检测影响脂肪沉积相关基因THRSP、CEBPα、PRKAG3和SREBF1的组织表达模式,期望寻找到影响阿勒泰羊臀脂沉积的关键基因,为进一步阐明绵羊臀脂沉积的分子调控机理提供参考。采集3只3岁健康阿勒泰母羊心、肝、脾、肺、肾、肌肉、皮下脂肪、尾脂8个组织,运用荧光定量PCR技术对上述基因在以上各个组织中的表达情况进行分析。结果表明,THRSP和CEBPα基因在阿勒泰羊肝、皮下脂肪和尾脂中的表达量显著高于其他组织(P<0.01),暗示此2个基因可能与脂肪的生成密切相关。PRKAG3基因在脂肪合成组织中低表达(P<0.01),提示其可能与脂肪的分解密切相关。研究结果为进一步阐明阿勒泰羊臀脂消长的生物学现象提供了科学思路。
The cold-resistance performance of Altay sheep is highly correlated with its tail fat （rump fat）. This paper aims to screen key genes （THRSP, CEBPa, PRKAG3, and SREBF1） which are related to adipose metabolism in tissues of Altay sheep. Those results will benefit the further understanding of molecular mechanism of adipose deposition in fat-tailed sheep. The expression profiles of four genes were studied in eight tissues （heart, liver, spleen, lung, kidney, muscle, subcutaneous adipose, fat of the tail） of 3 Altay ewes at age of 3 using SYBR Green RT-qPCR method. It revealed that two genes （THRSP, CEBPa） were highly expressed in liver, subcutaneous adipose and fat tail compared with other tissues （P d0.01）. It indicated that those two candidate genes are highly associated with adipogenesis in Altay sheep. PRKAG3 was lowly expressed in tissues for adipose synthesis （P《0.01）, which means that PRK- AG3 might be closely related with lipolysis. The results might expand understanding of molecular mechanism on adipose deposition and lysis in fat-tailed sheep breeds like Altay.
出处
《家畜生态学报》
北大核心
2016年第11期11-16,共6页
Journal of Domestic Animal Ecology
基金
NSFC新疆联合基金重点项目(U1130302)
中国农业科学院科技创新工程(ASTIP-IAS13)
国家肉羊产业技术体系专项(CARS-39)
关键词
阿勒泰羊
臀脂
尾脂
候选基因
表达谱
Altay sheep
rump fat
tail fat
candidate genes
expression profile
