摘要
为了揭示牦牛肾脏细胞在重金属胁迫下的毒理性影响,在培养出的肾脏细胞中加入不同浓度的重金属离子铅(Pb^2+)和镉(Cd^2+),提取总RNA,在逆转录酶的作用下生成c DNA,利用实时荧光定量技术检测牦牛肾脏细胞中金属硫蛋白mRNA的相对表达量。结果显示,将牦牛肾脏细胞中加入重金属浓度为0μmol/L的值设定为对照组,2^-ΔΔCt值设为1时,加入重金属铅的不同浓度0、5、10、20、30μmol/L的样品相对定量五种不同浓度的样品的2^-ΔΔCt分别为0.489、0.603、0.796、3.031;添加镉的五种不同浓度的样品的2^-ΔΔCt分别为5.280、3.530、8.110、11.630。通过差异性显著分析得出当重金属离子铅(Pb^2+)和镉(Cd^2+)添加10μmol/L时对肾脏细胞的生长有明显的抑制作用,相应的金属硫蛋白mRNA表达量较高,通过mRNA相对表达量的比较得出重金属镉的毒性比铅强,当重金属的添加量比较低时,重金属离子通过结合MT降低对肾脏细胞的毒害作用。
In order to reveal the yak kidney cells＇ poison rational influence under the stress of heavy metal, in the produce of kidney cells with different concentrations of heavy metal ions of lead （ Pb2＋） and cadmium （ Cd2＋ ）, metallothionein mRNA expression of yak kidney cells were detected by Real-time PCR.It showed that the value of sample after added five different concentrations of heavy metal lead were 0.489,0.603,0.796,3.031 and the value of sample of the five different concentrations of heavy metal cadmium were 5.28,3.53,8.11, 11.63, respectively, when adding different concentrations of heavy metal 0,5,10,20,30 μmol/L into the yak kidneycells.The result showed that it had obvious inhibitory effect on growth when added 10 t/mol/L Pb2＋ and Cd2＋ into the kidney cells,and the corresponding amount of mRNA expression of metallothionein was relatively high, and the toxicity of heavy metal Cd2＋ was stronger than Pb2＋ comparing to the relative expression of mRNA,and the toxicity of cells was reduced by combining MT when heavy metal content was relatively low.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第23期130-134,138,共6页
Science and Technology of Food Industry
基金
国家自然基金项目(31160215)
