摘要
为研究不同老芒麦(Elymus sibiricus)种质材料耐盐性表现,以筛选出耐盐品种,为耐盐老芒麦种质材料育种提供科学支撑,采用不同浓度梯度NaCl溶液(0.3%、0.6%、0.9%、1.2%、1.5%和1.8%)模拟盐胁迫,对9份来自全国不同地区的老芒麦种质材料进行耐盐处理,筛选出老芒麦萌发期适宜浓度,分别测定其萌发指标及幼苗叶片生理指标(脯氨酸、超氧化物歧化酶、丙二醛),分析不同浓度胁迫下指标的变化情况,并对9份老芒麦的耐盐性进行综合评价。结果表明,NaCl胁迫对老芒麦种子的相对发芽率、发芽指数均产生抑制影响,抑制了胚根与胚芽的生长,其叶片中超氧化物歧化酶活性、丙二醛及脯氨酸含量对盐胁迫浓度变化响应灵敏,0.9%NaCl盐浓度为合适的萌发期筛选浓度,根据隶属函数法进行综合评价,9份老芒麦材料耐盐性由强到弱依次为E03、E04、E08、E09、E07、E05、E06、E02和E01。
The aim of this study was to evaluate the salt resistence of different Elymus sibiricus germplasm and to screen varieties with salt resistance.The seeds of nine E.sibiricus were treated with different NaCl solutions of 0,0.3%,0.6%,0.9%,1.2%,1.5% and 1.8%,to select the best concentration of NaCl solution.The salt stress of nine accessions was studied in germination index and seedling leaf physiological indexes.The results of this study showed that the salt decreased the relative germination rate and relative germination index,inhibited the growth of radicle length and plumule length.It was obvious that the level of SOD enzyme activities,MDA,proline in the leaves at seedling stage were sensitive to the level of salt stress.The concentration of0.9% NaCl was suitable for screening salt tolerance in E.sibiricus at seed germination stage.According to the descending order of the comprehensive evaluation of salt resistance,the order of nine E.sibiricus accessions from high to low was E03,E04,E08,E09,E07,E05,E06,E02,E01.
出处
《草业科学》
CAS
CSCD
北大核心
2016年第11期2266-2275,共10页
Pratacultural Science
基金
"十二五"农村领域国家科技计划课题"淀粉类(小麦野生材料)种质遗传完整性新技术研究及监测"(2013BAD01B0105)
牧草种质资源保护项目(2015-167)
农作物种质资源保护项目(2015NWB037)
关键词
老芒麦
耐盐性
萌发
生理特性
隶属函数法
Elymus sibiricus
salt tolerance
seed germination
physiological characteristics
membership function method
