摘要
在油菜素甾醇(brassinosteroids,BRs)化合物中,油菜素内酯(brassinolide,BL)具有活性最高、广谱和无毒等显著特点,而且具有改良植物株型、提高抗逆性等功效。根系是植物吸收水分和矿质元素的主要器官,因此阐明油菜素内酯调控根系发育的遗传、生理和生化机制,有利于更有效地利用BRs激素,实现株型的定向设计。该研究利用叶面喷施的方法分析油菜素内酯对根系侧根、根毛发育的影响;利用植物显微技术分析油菜素内酯对根系侧根结构及发育的作用;利用高压液相色谱法检测油菜素内酯对根系内其他植物激素含量的影响;利用蛋白质组学技术鉴定受油菜素内酯调控的蛋白质,分析油菜素内酯调控根系发育的生化机制。研究表明,一定浓度的油菜素内酯促进种子根、侧根、根毛的发生;提高根系细胞分裂素和赤霉素含量;可能通过调控逆境相关蛋白质来提高植物的抗逆性。
In brassinosteroids （BRs） compounds, brassinolide （BL） has the highest activity, and other significant characteristics of broad spectrum and non-toxic, improving the plant type and resistance, and so on. Root is the main organ of plants absorbing water and nutrients, therefore we will clarify the genetic, physiological and biochemical mechanisms of BRs on the regulation of root development, for the more effective use of BRs hormone, architecture design and implementation of the directional. In present study, we analyze the influence of brassinolide on root development by foliar spraying method. We analyze of effects of brassinolide on root system development by plant microscopy technique. Then, we detect the influence of brassinolide on the other phytohormones content by high pressure liquid chromatography. We also identify proteins regulated by brassinosteroid by proteomics methods and analyze the physiological mechanism of brassinolide on the regulation of root system development. The results show that brassinosteroids of certain concentration can promote seed root and lateral root. Root cytokinin and gibberellin content increased through regulation of growth-related enzymes to improve plant development. BRs can promote the growth of rice seed root and lateral root and root hair. It may enhance the plant resistant through regulation stress related proteins.
出处
《中国细胞生物学学报》
CAS
CSCD
2016年第10期1191-1198,共8页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:31401759)资助的课题~~
