摘要
目的研究消退素D1对突出髓核所致根性神经痛的影响及内在机制。方法选取56只雄性Sprague-Dawley（SD）大鼠，按照随机数字表法分为假手术组、模型组、消退素Dl10ng组、消退素Dl100ng组，每组14只，建立非压迫性椎间盘突出症模型。术后1d、2d、3d，假手术组和模型组分别注射磷酸盐缓冲液（PBS）10μl，消退素D110ng组和消退素D1100ng组分别注射含有消退素D110ng和100ng的PBS10μl。于术前1d及术后连续7d分别检测各组大鼠术侧后肢50％缩足阈值（PWT）。术后7d，取大鼠脊髓组织经酶联免疫吸附测定法（ELISA）检测肿瘤坏死因子（TNF-α）、白介素1β（IL-1β）和白介素10（IL-10）的蛋白表达量，采用蛋白质印记Western blot法检测脊髓组织中细胞外信号调节激酶（ERK）核转录因子NF-κB／p65（NF—κB／p65）的含量变化。结果术后1d至7d，模型组大鼠50％PWT较假手术组明显降低（P〈0．05）；术后3d、4d、5d、6d、7d，消退素10ng组与模型组比较，50％PWT显著升高（P〈0．05）；术后2d、3d、4d、5d、6d、7d，消退素100ng组与模型组比较，50％PWT显著升高（P〈0．05）。与假手术组比较，模型组大鼠TNF-α和IL-1β的蛋白表达水平明显升高（P〈0．05），IL—10的表达水平明显降低（P〈0．05）；与模型组比较，消退素10ng组和消退素100ng组的TNF—α和IL-1β的蛋白表达水平明显降低（P〈0．05），IL-10的表达水平明显升高（P〈0．05），且消退素100ng组的蛋白含量下降更显著（P〈0．05）。与似手术组比较，模型组p-ERK、NF—κB／p65蛋白水平明显升高（P〈0．05）；与模型组比较，消退素10ng组和消退素100ng组p-ERK及NF-κB／p65蛋白水平明显降低（P〈0．05），且消退素100ng组降低的更明显（P〈0．05）。结论消退素D1通过蒯节炎症介质表达及p-ERK和NF—κB／p65通路的活性，促进神经根炎症的消退，为治疗腰
Objective To investigate the analgesic effect of Resolvin D1 （ RvD1 ） on radicular pain induced by herniated nucleus pulposusand its underlying mechanism. Methods Fifty-six male rats were randomly divided intoa sham group, a model group, a 10 ng group anda 100 ng group, each of 14. The rat model of non-compressive lumber disc herniation was established in all exeept the sham group. The former two groups were then injected with 10 μl of phosphate buffer solution （PBS） while the latter 2 groups were injected with 10 μl of PBS containing 10 and 100 ng of RvDI respectively daily for three successive days. The rats＇ 50% paw withdrawal threshold （PWT） was evaluated 1 day before and on 7 successive days after surgery. On day 7 the rats＇ spinal cords were removed to assess the expression of tumor necrosis factor-α（TNF-α） , interlukin-1β（IL-1β） and interlukin-10 （IL-10） using ELISA methods. The levels of ERK and NF-nB/p65 were measured using Western blotting. Results Theaverage 50%PWT of the model group decreased significantly from day 1 to clay 7 compared with the sham group, hut was significantly lower thanthe RvDI 10 ng group from day 3 to day 7. Moreover the 50%PWT in the RvD1 100 ng group increased signifieantlyfrom day 2 to day 7 compared with the model group （P〈0.05）. The average expression of both TNF-α and IL-1β of the model group was upregulated significantly and that of IL-10 decreased significantly compared with the sham group. Compared with the model group, the expression of TNF-α and IL-1β dec.teased significantly （P〈0.05） and the level of IL-10 was significantlyup-regulated （P〈0.05） both in the RvDI 10 ng group and 100 ng group. Moreover, the changes were larger in the RvDI 100 ng group （P〈0.05）. Compared with the sham group, the levels of p-ERK and NF-KB/p65 in the model group were significantly up-regulated （P〈 0.05）. Compared with the model group, intrathecal injection of RvDI （ 10 ng or 100 ng） significantly decreased the expression
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2016年第11期819-824,共6页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金（81271232）
山东省科技发展计划（2014GSF118130）
国家临床重点专科建设项目经费资助
