摘要
目的·应用血管内穿刺法建立蛛网膜下腔出血(SAH)后早期脑损伤兔模型并评估模型的有效性和可靠性。方法·共采用59只新西兰兔进行实验,其中假手术组(实施颈内动脉超选择造影但未穿刺)5只,应用数字减影血管造影(DSA)对54只新西兰兔行血管内穿刺建模,30只存活兔随机分为6 h、24 h和72 h组(n=10)进行后续研究。采用颅内压(ICP)监测仪观察模型制作过程中动物的ICP变化情况,SAH评分和神经功能评分用于评估出血量和神经功能障碍严重程度。术后各时间点行TUNEL和FJB染色观察皮层和海马组织变性坏死和凋亡情况。结果·SAH兔模型制作成功率为55.6%(30/54)。血管内穿刺法可以引发不同程度颅内出血和神经功能障碍(P=0.000),同时可以造成皮层和海马组织变性坏死和凋亡。结论·应用血管内穿刺法建立的蛛网膜下腔出血兔模型成功模拟了早期脑损伤的颅内血流动力学和病理生理学改变,对于研究蛛网膜下腔出血后早期脑损伤的发生机制具有重要意义。
Objective · To build a rabbit model of early brain injury （EBI） after subarachnoid hemorrhage （SAH） with endovascular puncture and to assess the validity and reliability of the model. Methods · A total of 59 New Zealand rabbits were used. Five of them were assigned to the sham operation group （undergoing super choice imaging for internal carotid artery without puncture） and other fifty-four underwent endovascular puncture under the guidance of digital subtraction angiography （DSA）. The 30 survived rabbits were randomly assigned to the 6 h, 24 h, and 72 h groups （n=10） for subsequent study. The intracranial pressure （ICP） monitor was used to observe the intraoperative ICP of the animals. SAH scores and neurological scores were used to assess the amount of bleeding and neurological dysfunction. TUNEL and FJB staining were used at three postoperative time points to evaluate the degeneration/necrosis and apoptosis in cortical and hippocampal tissues. Results · The successful rate of the SAH rabbit model was 55.6% （30/54）. The endovascular puncture resulted in different degrees of intracranial hemorrhage and neurological dysfunction （P=0.000）, which caused degeneration/necrosis and apoptosis in cortical and hippocampal tissues. Conclusion · The rabbit model of SAH with endovascular puncture successfully simulates changes in haemodynamics and pathophysiology for EBI and is important for studying the mechanisms of EBI after SAH.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2016年第11期1543-1548,共6页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(81471333)
上海交通大学医学院附属仁济医院种子基金(RJZZ13-007)~~
关键词
蛛网膜下腔出血
早期脑损伤
血管内穿刺
动物模型
subarachnoid hemorrhage
early brain injury
endovascular puncture
animal model
