摘要
【目的】研究2014年新疆地区猪繁殖与呼吸综合征病毒(PRRSV)遗传变异情况,监测PRRSV在新疆流行情况,为新疆的PRRSV血清学检测提供帮助。【方法】采用RT-PCR方法对分离自新疆地区的PRRS疑似病料进行ORF7基因扩增和测序,分析PRRSV ORF7基因变异情况。【结果】检测到18株PRRSV毒株具有ORF7碱基突变,均属北美洲型,与国外的参考毒株和国内的参考毒株有着不同的亲缘关系。其中,XJzx4、XJzx12与国外的参考毒株01NP1.2、PA8、VR-2332、LMY亲缘关系较近。除XJzx1、XJzx14外,其余毒株与内地毒株BJsy06、GD、HB-1sh2002、Hu N、JXA1、LN、NX06、SX2009亲缘关系较近,推断由内地毒株演化而来。【结论】新疆本地流行猪繁殖与呼吸综合征毒株是来自国内外不同地区毒株,ORF7基因在新疆具有不同的遗传变异类型。没有检测到新疆猪繁殖与呼吸综合征病毒ORF7基因有缺失型突变。
【Objective】To study porcine reproductive and respiratory syndrome virus（ PRRSV） genetic variation to monitor the prevalence of PRRSV in Xinjiang and provide assistance to PRRSV serology.【Method】We used RT-PCR method for the separation of 18 PRRSV ORF7 gene which was amplified,cloned and sequenced to analyze the ORF7 gene variation【Results】The 18 PRRSV ORF7 sequence mutation type belonged to North America type. They had different genetic strain relations with the international standards and national standards strains. XJzx 4 and XJzx 1 had a closer genetic relationship with the international standard strains01NP1. 2,PA8,VR-2332 and LMY. Except XJzx1,XJzx14,the rest strains had close relationships with the mainland strains BJsy06,GD,HB-1sh 2002,Hu N,JXA1,LN,NX06 and SX2009,so it could be inferred that they evolved from the strains in China's Mainland. 【Conclusion】It is hypothesized that local pop PRRSV strains are derived from different regions. We have not detected PRRSV ORF7 gene deletion mutation in Xinjiang.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2016年第11期2149-2156,共8页
Xinjiang Agricultural Sciences
基金
国家自然科学基金项目"绵羊新抗微生物多肽基因的筛选
酵母表达及抗性分析"(30960276)
国家自然科学基金项目"新疆不同品种绵羊抗菌肽NK-Lysin多态性与抗肿瘤活性关系研究"(31360589)~~
