摘要
以MHCⅡ类基因第二外显子为分子标记，利用限制性内切酶分析法和序列分析法对8个布氏田鼠Lasiopodomys brandtii地理种群进行遗传多样性分析。结果显示，8个布氏田鼠地理种群的MHCⅡ类基因第二外显子酶切共检测到6个等位基因，定义21种单倍型，其中有3个单倍型为不同区域种群共享，经卡方检验，6个酶切多态性位点上基因型频率不符合Hardy—Weinberg平衡；序列分析显示，在261bp的核苷酸序列中，有57个变异位点，单倍型多样性为0．7465～0．8733、核苷酸多样性为0．00606～0．01655。谱系分析得到3个稳定的分支：锡林浩特、二连浩特、东乌珠穆沁旗和西乌珠穆沁旗4个种群形成一个单元分歧的进化分支（A区域），新巴尔虎左旗、陈巴尔虎旗和新巴尔虎右旗3个种群成一个单元分歧的进化分支（B区域），而位于浑善达克沙漠南部的正镶白旗种群形成一个独立单元分歧的进化分支（C区域），分别与采集的地理种群相吻合。AMOVA分析结果表明，区域类群间的遗传变异占总变异比率的64．08％，区域内种群间占7．78％，种群内占28．14％。除正镶白旗种群外，布氏田鼠种群具有较丰富的遗传多样性，遗传变异主要发生在区域类群之间和种群内。
The genetic diversity of 8 geo-populations of Lasiopodomys brandtii were analyzed using PCR-RFLP and sequence analysis based on MHC class Ⅱ exon 2. The results showed that 6 alleles, 21 haplotypes and 13 restriction enzyme polymorphic loci were detected, and 3 haplotypes were shared by all the populations. The Chi-square test results suggested that the 8 populations of L. brandtii in the present study did not follow with the Hardy-Weinberg equilibrium at six restriction enzyme polymorphic loci. Sequence analysis showed that there were 57 bases mutations. Haplotype diversity （H） and nu- cleotide diversity （π0） were 0. 746 5 - 0. 873 3 and 0. 006 06 - 0. 016 55, respectively. Phylogenetic analyses suggested that the sampled individuals were clustered into three clades ： populations of Xilinhaote, Erlianhaote, Dongwuqi, and Xiwu- qi were clustered as Zone A; populations of Xinbaerhuzuoqi, Chenbaerhuqi and Xinbaerhuyouqi were clustered as Zone B; while the Zone C was comprised of the sole population from Zhengxiangbaiqi. And this clustering pattern was consistent with their geographical distributions. The result of AMOVA analysis showed that regional genetic variation was accounted for 64. 08% of the total, intraregional population variation was accounted for 7.78%, and individual variation was accounted for 28. 14%. There was a rich genetic diversity among the populations of L. brandtii except Zhengxiangbaiqi population. The majority of genetic variation occurred among groups and within populations.
出处
《四川动物》
北大核心
2016年第6期818-824,共7页
Sichuan Journal of Zoology
基金
安徽省高校学科(专业)拔尖人才学术资助重点项目(gxbj ZD2016108)
安徽省教育厅自然科学基金重点项目(KJ2012Z406)
国家重点基础研究发展规划项目(9732007BC109103)
国家自然科学基金项目(31101806)
