摘要
目的研究多发性骨髓瘤(MM)细胞中B细胞活化因子(BAFF)及B细胞成熟抗原(BCMA)的表达及作用,JNK信号通路的表达及活化情况,探讨JNK信号通路活化与BCMA表达间的相互作用。方法采用实时荧光定量(QRT)-聚合酶链式反应(PCR)检测MM患者及健康对照者单核细胞(PBMCs)中BAFF及其受体BCMA、TACI、BAFF-R的表达。采用Western Blot方法检测MM细胞中BCMA蛋白的表达及信号通路蛋白ERK、p-ERK、JNK、p-JNK、p38及p-p38的表达及活化情况;采用WST-1法检测MM细胞的增殖与存活。结果 MM患者PBMCs中BAFF及BCMA的表达显著高于健康对照者。人BAFF重组体(rhBAFF)促进MM细胞的增殖,Si-BCMA可抑制rhBAFF对MM细胞的增殖作用。除了ERK、JNK及p38的表达外,MM细胞中还有活化蛋白p-JNK的表达。Si-BCMA可下调p-JNK的表达,JNK信号通路抑制剂可下调p-JNK及BCMA的表达。结论 MM细胞中,BAFF通过BCMA促进MM细胞增殖。JNK信号通路活化与BCMA表达相互作用,共同促进MM细胞的增殖与存活。
Objective To investigate the expression and role of B-cell activating factor（BAFF）and B-cell maturation antigen（BCMA）in multiple myeloma（MM）cells,expression and activation of JNK signal pathway and to explore the interaction between JNK signal pathway activation and BCMA expression.Methods The expression of BAFF and its receptor BCMA,TACI and BAFF-R in peripheral blood mononuclear cells（PBMCs）of MM patients and healthy controls were detected by the quantitative realtime fluorescence PCR（QRT-PCR）.Western blot was adopted to detect the expression of BCMA protein and the expression and activation of signal pathway protein ERK,p-ERK,JNK,p-JNK,p38 and p-p38.MM cell proliferation and survival were measured by WST-1assay.Results The expression of BAFF and BCMA in PBMCs of MM patients was significantly higher than that of healthy controls.Recombinant human BAFF（rhBAFF）promoted the proliferation of MM cells,while Si-BCMA could restrain the proliferative effect of rhBAFF on MM cells.In addition to the expression of ERK,JNK,p38,p-JNK was also expressed in MM cell lines.SiBCMA could down-regulate the expression of p-JNK,while the JNK pathway inhibitor could down-regulate the expression of pJNK and BCMA.Conclusion BAFF promotes the proliferation of MM cells via BCMA.The interaction between JNK signal pathway activation and BCMA expression commonly promotes the proliferation and survival of MM cells.
出处
《国际检验医学杂志》
CAS
2016年第22期3117-3119,3122,共4页
International Journal of Laboratory Medicine
