摘要
以油酸、聚乙二醇、柠檬酸钠和3-氨丙基三乙氧基硅烷(APTES)为改性剂,采用化学共沉淀法制备4种改性磁性微球.通过粒度测定(PSD)、X射线衍射分析(XRD)、傅里叶红外光谱分析(FTIR)、热重分析(TGA)、磁强度测定(VSM)对各种磁性微球进行表征,并对比各微球固定化铁还原菌后还原Fe(Ⅲ)EDTA的性能,最终确定最佳的磁性微球改性条件.结果表明:APTES改性的Fe_3O_4磁性微球固定化铁还原菌效果优于其他改性微球,其最佳条件为APTES投加量8 mL,1 mg铁还原菌需1.5g磁性微球进行固定化;APTES-Fe3O4固定化铁还原菌后连续使用5次,其Fe(Ⅲ)EDTA还原效率仍可保持在90%以上.
The modifiers of oleic acid,polyethylene glycol,sodiumcitrate and 3-ammonia propyl triethoxy silane（APTES）were applied to modify the Fe3O4 magnetic microspheres by chemical co-precipitation method.The magnetic microspheres were characterized by PSD,XRD,FTIR,TGA and VSM,and reduction rates of Fe（Ⅲ）EDTA by different magnetic microsphere immobilized iron reducing bacteria were compared,to determine the optimal preparation conditions.The results showed that the Fe（Ⅲ）EDTA reduction rate of the ironreducing bacterium immobilized on the APTES-Fe3O4 microspheres was higher than that of the other microspheres.The optimal preparation conditions for the APTES-Fe3O4 microspheres were determined as follows：8mL of APTES,and 1.5g magnetic microspheres for immobilization of 1mg iron-reducing bacteria.More importantly,the reduction efficiency still maintained 90% of its initial reduction ability after the 5th reused cycle.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2016年第6期720-724,共5页
Journal of Huaqiao University(Natural Science)
基金
国家自然科学基金资助项目(21077035
NCET-11-0851)
关键词
磁性微球
改性
3-氨丙基三乙氧基硅烷
铁还原菌
固定化
magnetic microsphere
modify
3-ammonia propyl triethoxy silane
iron reducing bacteria
immobilized
