摘要
Rim101是一个具有锌指结构的转录因子,在调控酿酒酵母细胞耐受碱性和高盐环境、钙离子稳态、细胞分裂以及硒毒性方面起作用。前人研究结果显示,细胞周期依赖性激酶基因PHO85的缺失,导致Rim101蛋白在细胞核内积累。为了探索Rim101亚细胞定位的新调节因子,通过荧光显微镜技术对酿酒酵母细胞基因组中编码磷酸酶的73个非必需基因缺失株和编码激酶的139个非必需基因缺失株进行了筛选,发现编码磷脂酰肌醇磷酸(Ptd Ins P)的磷酸酶Sac1调控Rim101的亚细胞定位。
Rim101 is a zinc-finger structured transcriptional factor that functions in the response of Saccharomyces cerevisiae cells to both alkaline and salt stress,calcium homeostasis,cell differentiation and selenite toxicity. Previous studies have shown that deletion of the cyclin-dependent kinase gene PHO85 leads to the nuclear accumulation of Rim101. In order to identify new regulatory factors in the subcellular localization of Rim101,deletion mutants of nonessential genes encoding 73 phosphatases or 139 kinases was screened through a fluorescent microscopy approach. It was found that the encoded thosphatidylinositol phosphate（ Ptd Ins P） phosphatase Sac1 regulates the subcellular localization of Rim101.
出处
《微生物学杂志》
CAS
CSCD
2016年第5期9-14,共6页
Journal of Microbiology
基金
国家自然科学基金项目(81371784
81571966
31301021)
江南大学自主研究计划重点项目(JUSRP51313B)
江苏市农业支撑项目(BE2014306)
