摘要
目的评价不同时间点洗胃（gastriclavage，GL）清除猪胃内百草枯（paraquat，PQ）的效果，以及对血浆PQ浓度的影响。方法将17头健康雌性家猪按随机数字表法分为PQ组（n=6）、PQ＋1hGL组（／7,=6）、PQ＋6hGL组（n=5）。所有动物按60mg／kg经胃管注入40％PQ原液，PQ4-1hGL组和PQ＋6hGL组分别在1h和6h经胃管给予20L温水单次洗胃，监测生命体征及血药浓度并持续观察24h，LC-MS法测量血浆及HPLC法测量胃洗出液中的百草枯浓度。结果PQ组、PQ＋6hGL组和PQ＋1hGL组的24h存活率分别为66．7％、80％、100％；血PQ峰浓度（μg／mL）分别为（5．12±3．38）、（3．99±1．24）、（1．96±1．83）；PQ＋1hGL组和PQ＋6hGL组洗出液的PQ含量占总摄入量的（24．46±6．49）％掷．（30．72±9．86）％（P〉0．05）；前10L和后10L的洗出液中PQ的清除比例：PQ＋1hGL组为（20．44±5．59）％伽．（4．02±1．82）％（P〈0．01），PQ＋6hGL组为（26．17±7．19）％铘．（7．03±4．29）％（P〈0．01）。结论1h和6h洗胃均能有效清除PQ，减少24h病死率；尽早洗胃（1h）能更明显降低血浆PQ峰浓度；洗胃液在后1／2体积（后10L洗出液）时清除百草枯的能力明显下降。
Objective To evaluate the elimination effect of gastric lavage （GL） in the swine model of paraquat （PQ） poisoning by using the determination of PQ plasma concentration. Methods A total of 17 healthy female domestic swine were randomly divided into PQ group （ n = 6）, PQ ＋ 1 h GL group （ n = 6 ） and PQ ＋ 6 h GL group （ n = 5 ）. A dose of 60 mg/kg PQ in 40% PQ solution was administered in all animals through gastric tube. Swine of PQ ＋ 1 h GL group and PQ ＋ 6 h GL group were treated with gastric lavage by using 20 L warm water at 1 h and 6 h after ingestion of PQ, respectively. Vital signs and PQ plasma concentration were monitored continuously for 24 h. LC-MS device was used to measure the PQ concentrations in lavage fluid and plasma, and HPLC was performed to measure the fluid drug concentration. Results The 24 h survival rates of PQ group, PQ ＋ 6 h GL group and PQ ＋ 1 h GL group were 66.7%, 80% and 100%, respectively. The peak plasma PQ concentration in PQ group was （5. 12 ±3.38） μg/mL, inPQ＋6 h GLgroupwas （3.99＋1.24） μg/mL, andinPQ＋l h GLgroupwas （1.96± 1.83 ） μg/mL. The percentage of the total amount of PQ ingested in the gastric lavage fluid in PQ ＋ 1 h GL group was （24. 46 ± 6. 49） % and in PQ ＋ 6 h GL group was （30. 72 ± 9. 86） % （P 〉 0. 05 ）. Alternatively,the gastric lavage process was divided into two successive procedures with 10 L warm water in each instead of 20 L water it~ one procedure . The content of PQ in lavage fluid from the first 10 L warm water was （20. 44 ＋ 5.59） % and that from the second 10 L warm water was （4. 02 ＋ 1.82） % in PQ ＋ 1 h GL group （ P 〈 0. 01 ）, whereas （26. 17 ＋ 7.19）% PQ in gastric lavage fluid from the first 10 L warm water and （7.03 ＋ 4. 29）% PQ in gastric lavage fluid from the second 10 L warm water, in PQ ＋6 h GL group （P 〈0.01 ）. Conclusions Gastric lavage process carried out in two successive procedures with 10 L warm water in each can eliminate PQ effecti
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2016年第11期1381-1385,共5页
Chinese Journal of Emergency Medicine
基金
国家自然科学基金（81571875
81671899）
江苏省科技厅重点病种规范化诊疗研究（BL2014088）
江苏省自然科学基金（BK20131444）
江苏省高校优势学科建设工程资助项目（J）（10231801）
关键词
百草枯
洗胃
药物浓度
清除率
Paraquat
Gastric lavage
Drug concentration
Clearance ratio
