摘要
目的：观察含笑内酯（MCL）对三阴性乳腺癌（TNBC）细胞顺铂（DDP）化疗敏感性的影响，并分析其可能的机制。方法将 TNBC 细胞分为 MCL 组、DDP 组、DDP ＋MCL 组，分别加入 MCL、DDP 和 DDP ＋MCL；另设空白对照组，加入等体积培养基。培养24 h 后，采用 CCK8法测算细胞存活率，计算联合指数（CI）评价两药是协同（CI＜0．9）或相加（CI 0．9～1．1）关系；采用流式细胞仪检测凋亡细胞，计算细胞凋亡率；采用酶标仪检测细胞上清液中的谷胱甘肽。结果 MCL（2．5～10μmol／L）、DDP（1．25～5μmol／L）联合作用 TNBC 细胞时，CI ＜0．85；空白对照组、MCL 组、DDP 组、MCL ＋DDP 组细胞凋亡率分别为4．3％±1．3％、7．6％±2．0％、13．4％±4．3％、37．0％±5．7％，MCL ＋DDP 组细胞凋亡率明显高于其他3组（P 均＜0．01）。空白对照组、MCL 组、DDP 组、MCL ＋DDP 组细胞上清液中谷胱甘肽水平分别为（43．54±3．71）、（35．85±2．67）、（50．33＋3．16）、（38．46±1．74）mmol／mg，MCL组、MCL ＋DDP 组〈空白对照组〈 DDP 组，P 均＜0．05；MCL 组、MCL ＋DDP 组比较，P ＞0．05。结论 MCL 可通过降低细胞内谷胱甘肽水平，从而增强 TNBC 细胞对 DDP 的敏感性。
Objective To observe the effect of Micheliolide （MCL）on chemosensitivity of triple negative breast canc-er （TNBC）cells to cisplatin （DDP）and the related mechanisms.Methods TNBC cells were randomly divided into MCL group,DDP group and DDP ＋MCL group,which were respectively added with MCL,DDP and DDP ＋MCL.Another blank control group was added with equal volume of culture medium.The cell viability was measured by CCK8,the CI was calculated,apoptotic cells were calculated by flow cytometry as well as the apoptosis rate,and glutathione （GSH）in super-natant was detected by ELISA.Results MCL（1-10 μmol/L）combined with DDP （1-5 μmol/L）showed an obvious syner-gistic effect（CI 〈0.85）,the apoptosis rates were 4.3% ±1.33%,7.6% ±2.02%,13.4% ±4.26% and 37.0% ±5.74%in the control,MCL,DDP and MCL ＋DDP groups,respectively;the apoptosis rate of the MCL ＋DDP group was higher than that of the MCL group and DDP group （all P 〈0.05）.The intracellular GSH levels were （43.54 ±3.71）,（35.85 ±2.67）, （50.33 ＋3.16）,and （38.46 ±1.74）mmol/mg in the control,MCL,DDP and MCL ＋DDP groups,respectively.The in-tracellular GSH level：MCL group,MCL ＋DDP group 〈blank control group 〈DDP group,all P 〈0.05;and no significant difference was found between the MCL group and MCL ＋DDP group,P 〉0.05.Conclusion MCL enhances the sensitivity of TNBC cells MDA-MB-231 to cisplatin by decreasing intracellular GSH levels.
出处
《山东医药》
CAS
北大核心
2016年第42期14-16,共3页
Shandong Medical Journal
基金
教育部高等学校博士学科点专项科研基金资助项目(20131202120003)
天津市抗癌重大专项攻关计划(12ZCDZSY16200)
关键词
三阴性乳腺癌
含笑内酯
顺铂
耐药
triple negative breast carcinoma
micheliolide
cisplatin
drug resistance
