摘要
采用盆栽试验与称重控水法,将土壤相对含水量分别控制在田间最大持水量的100%、91.68%、82.85%、60.00%、41.86%和21.28%,并在这6个不同的土壤相对含水量条件下,分别设添加苯菌灵(杀真菌剂)(低AMF)和不添加苯菌灵(高AMF)处理,研究干旱胁迫下AMF对极小种群野生植物云南蓝果树幼苗生长和光合特征的影响,揭示云南蓝果树濒危的微生物学机制,为云南蓝果树保护措施的制定与实施奠定基础。结果表明,添加苯菌灵处理显著降低了不同水分条件下的AMF侵染率,说明试验中AMF处理的实生苗在生长和光合特征上的差异是苯菌灵处理下侵染率下降导致的;随着干旱胁迫的加剧,云南蓝果树幼苗的根部AMF侵染率显著降低、叶面积等生长指标和净光合速率(Pn)等光合参数都发生显著变化;高AMF处理可以显著增加水分充足和轻度干旱胁迫条件下云南蓝果树幼苗的大部分生长指标和光合参数,而对重度胁迫下的云南蓝果树幼苗没有显著影响,说明重度干旱胁迫对其影响大于AMF的影响;另外,整合了可塑性指数分析和隶属函数分析两种方法对其抗旱性进行评价,云南蓝果树幼苗基本上无法通过调节形态和光合能力来适应水分环境的变化,但是高AMF处理可使云南蓝果树幼苗具有较强的可塑性和更强的抗旱性。实验结果为云南蓝果树的科学保育及种苗繁育提供了理论依据。
Among various environmental stresses, drought stress has become a critical problem worldwide due to its dramatic effects on plant growth and physiology, and recently, this has attracted much attention. Arbuscular mycorrhizal fungi （AMF） have previously been reported to afford greater resistance to drought stress in some plant species. The aim of this study was to determine the impacts of AMF on growth and photosynthetic through addition （Low AMF） or non-addition （High AMF） of the fungicid characteristics of Nyssa ytmnanensis seedlings e benomyl at each water treatment. We further aimed to elucidate drought resistance and associated microbiological mechanisms of this endangered plant species, which would help to establish and implement protection measures. In this study, a pot experiment was conducted to control the soil water content, and 100, 91.68, 82.85, 60.00, 41.86, and 21.28% of field moisture capacity were set. The seedlings were subjected to different watering regimes and measurements were made at the end of the experiment to investigate the and photosynthetic characteristics of N. yunnanensis seedlings growth Plasticity index analysis and principal component analysis of 18 indices of N. yunnanensis were integrated and analyzed using the membership function method to evaluate synthetic drought resistance. These variables provide a theoretical basis for a better understanding of the mechanism of AMF on the tolerance of N. yunnanensis seedlings to drought stress. The results showed that the AMF colonization rate of N. yunnanensis roots significantly decreased under ＂Low AMF＂ treatments, indicating that differences in the growth and photosynthetic characteristics of AMF-treated seedlings were caused by a reduction in the rate of AMF colonization in N. yunnanensis roots. With the intensification of drought stress, the AMF colonization rate of N. yunnanensis roots significantly decreased and changed both growth parameters, including leaf area, and photosynthetic characteristics, including net p
出处
《生态学报》
CAS
CSCD
北大核心
2016年第21期6850-6862,共13页
Acta Ecologica Sinica
基金
国家自然科学基金项目(31460119)
国家林业局珍稀濒危物种野外救护与繁育项目(2014YB1004
2015YB1021)
云南省应用基础研究青年项目(2013FD075)
关键词
丛枝菌根真菌
干旱胁迫
生长指标
光合特征
云南蓝果树
arbuscular mycorrhizal fungi （AMF）
drought stress
growth parameters
photosynthetic characteristics
Nyssa yunnanensis
