摘要
目的观察活血通络方在糖尿病胃轻瘫大鼠模型中的治疗效果及对胃动素、胃泌素水平的影响。方法选择90只健康雄性Wistar大鼠,随机分为对照组、吗丁啉治疗组、模型组、中药1组(含丹参、川芎)、中药2组(含水蛭、地龙)、中药3组(活血化瘀通络方,含丹参、川芎、水蛭、地龙)。各组在造模时灌胃给药,给药剂量:正常对照组服用1.5ml/100 g生理盐水,模型组摄入1.5 ml/100 g生理盐水,中药1组给予丹参、川芎1.2 g/(kg·d)灌胃,中药2组给予水蛭、地龙1.2 g/(kg·d)灌胃,中药3组给予丹参、川芎、水蛭、地龙合方1.2 g/(kg·d)灌胃,周期为8周。购入的实验大鼠放入SPF级的饲养条件下进行稳定,7 d后每只大鼠均禁食12 h,造模,在24 h、36 h、72 h测定90只大鼠非空腹尾静脉血糖值,将血糖值长期稳定于16.8 mmol/L的60只大鼠作为研究对象,平均分入各组中,每组12只。另外选择12只健康大鼠入对照组中,与其他5组操作方式相似,以等量消毒生理盐水对大鼠进行腹腔注射。共接受8周治疗,在最后给药时检测各组大鼠血糖值、体重,24 h禁食后各组均经口灌胃30 min后,空腹取眶静脉血3 ml,放于含有10%乙二胺四乙酸二钠30μl和抑肽酶60μl试管内,4℃下离心后取血浆放于-20℃冰箱内保存测定胃动素及胃泌素水平。结果(1)各组给药8周后,均出现了动物死亡,以模型组大鼠的死亡率为最高,存活率仅为58.33%(7/12)。其他各个组的存活率显著高于模型组(P<0.05)。(2)与模型组相比较,对照组、中药各组、治疗组大鼠的胃动素、胃泌素水平明显降低;与中药1组、2组大鼠比较,中药3组胃动素、胃泌素水平明显升高(P<0.05)。结论活血通络方、中药1组、2组及吗丁啉治疗组均可以相应地提高糖尿病胃轻瘫大鼠的存活率,通络方对糖尿病胃轻瘫大鼠血糖具有一定的改善作用,能够降低血浆胃动素、胃泌素水平,提升大鼠胃肠推进,�
Objective To observe the effect of blood circulation Tongluo Recipe on diabetic rat model of gastric paresis and the effect of the changes of rootilin and gastrin. Methods A total of ninety healthy male Wistar rats were chosen and randomly divided into the control group, domperidone treatment group, model group, traditional Chinese medicine 1 group, （including Salvia, Chuanxiong）, Chinese medicine 2 group （including leech, earthworm）, traditional Chinese medicine 3 group （blood circulation Tongluo Prescription containing Salvia, Chuanxiong, leech, earthworm）. The rats were taken with stable feeding for 7 days after purchasing, fasting 12 h. The tail vein of non - fasting blood glucose level of 90 rats 24 h, 36 h, 72 h after injection were measured. 60 rats with blood sugar level steady at 16.8 mmol/L were chosen, average into each group, 12 cases in each group. 12 healthy rats were chosen as the control group, similar operation with the other five groups, with the same amount of sterilized saline rats injected intraperitoneally. After 8 weeks of treatment, after the last administration, blood glucose values and body weight of the rats were detected. Fasting after 24 h, after completion of 30 min, fasting orbital venous blood 3 ml, placed containing 10% EDTA -2Na 30 μl and 60μl tubes, at 4℃ after centrifugation, the plasma placed in -20℃ refrigerator for saving and determination motilin and changes of gastrin indicators. Results ①Medication after 8 weeks in each group, there were deaths, and the mortality of the model group were the highest, and the survival rate was 58.33% （7/12）. The survival rate in the each of other groups was significantly higher than that of the model group （ P 〈 0.05 ）. ②Compared with the model group, the changes of motilin and gastrin indicators of the control group, Chinese medicine groups, treated group were significantly reduced. Compared with the traditional Chinese medicine 1 group and 2 group, the motilin and gastrin indicator of 3 group was significa
出处
《临床和实验医学杂志》
2016年第22期2188-2191,共4页
Journal of Clinical and Experimental Medicine
基金
河北省中医药管理局科研课题(编号:2014212)
