摘要
目的：了解我院泛耐药鲍曼不动杆菌（PDR—AB）碳青霉烯酶OXA-23基因及介导氨基糖苷类高水平耐药的ArmA甲基化酶基因的存在状况，为有效的临床治疗和医院感染控制提供实验室依据。方法：收集我院2013-2015年从ICU病区分离的泛耐药鲍曼不动杆菌共60株，经VITEK2-Compact全自动细菌鉴定仪进行细菌鉴定和药敏试验，采用PCR检测OXA-23基因及AnnA甲基化酶基因，并对其扩增产物进行基因测序。结果：60株泛耐药鲍曼不动杆菌所携带的OXA-23基因阳性率83．3％（50／60），ArlnA基因阳性率为98．3％（59／60）。同时携带2种基因型有50株。结论：我院ICU分离的泛耐药鲍曼不动杆菌中，广泛存在着OXA-23基因及AmL4甲基化酶基因，应引起临床医生高度重视，防止在院内其他的临床科室流行传播。
Objective： To understand the existence status of the carbapenem resistant OXA-23 gene and ArmA methylation gene mediated by high level resistance of amino glycosides of pandrug-resistant acinetobacter baumannii （PDR.-AB） in our hospital and provide laboratory evidence fbr clinical treatment and hospital infection control.Methods： A total of 60 strains of PDR- AB isolated from ICU ward in our hospital during 2013-2015 were collected. Bacterial identification and drug sensitivity test were performed by 2-Compact VITEK automatic bacterial identification instrument. OXA--23 gene and ArmA gee were detected by PC1K, and the amplified products were sequenced.Results： The positive rate of OXA-23 gene carried by （~0 strains of PDIK-AB was 83.3% （50/60） , and the positive rate of ArmA gene was 98.3% （59/60） . There were 50 strains with these two genotypes. Conclusion： OXA-23 gene and ArmA methylation enzyme gene are widely existed in PDR--AB isolated fi＇om ICU award of our hospital. Clinicians should pay more attention to prevent the spread of other clinical departments in hospital.
出处
《医学检验与临床》
2016年第6期22-24,38,共4页
Medical Laboratory Science and Clinics
