摘要
目的：探讨汉黄芩素对胃癌细胞SGC7901凋亡、侵袭迁移及Wnt/β-连接素（β-catenin）信号通路的影响。方法：常规培养3种常见胃癌细胞株（SGC7901、BGC-823及MKN-45）至对数生长期,采用终浓度0、20、50、100、200μmol/L汉黄芩素处理24、48、72、96 h后采用四甲基偶氮唑盐比色法检测细胞增殖情况,分别采用Annexin V-FITC/PI双染流式细胞术、划痕实验及Transwell细胞侵袭实验检测各浓度汉黄芩素作用24、48 h后SGC7901细胞的凋亡率及迁移、侵袭能力,Western blotting检测各浓度汉黄芩素作用48 h后SGC7901细胞Wnt/β-catenin信号通路中β-catenin及下游靶分子C-myc、细胞周期素D1（Cyclin D1）的蛋白水平。结果：汉黄芩素在20-200μmol/L范围内可呈浓度和时间依赖性抑制SGC7901、BGC-823及MKN-45细胞增殖。与汉黄芩素0μmol/L组比较,各浓度汉黄芩素作用24、48 h后SGC7901细胞凋亡率均升高,迁移距离和穿膜细胞数均降低（P〈0.05）;除汉黄芩素20μmol/L组的β-catenin水平外,其余浓度SGC7901细胞β-catenin、C-myc及Cyclin D1蛋白水平均低于0μmol/L（P〈0.05）,其作用呈量-效关系。结论：汉黄芩素对胃癌细胞的增殖有抑制作用,同时可诱导凋亡和抑制细胞侵袭和迁移,其机制可能与抑制Wnt/β-catenin通路激活有关。
Objective： To explore the effects of wogonin on the apoptosis,invasion,migration and Wnt / β-catenin signaling pathway of gastric cancer cells SGC7901. Methods： Three common gastric cancer cell lines（ SGC7901,BGC-823 and MKN-45） were conventionally cultured to logarithmic growth. The cell proliferation was detected by MTT assay after treatment with different concentrations of wogonin（ 0,20,50,100 and 200 μmol / L） for 24,48,72 and 96 h. The apoptosis rate,migration and invasion ability of SGC7901 cells were detected by Annexin V-FITC / PI annexin double staining flow cytometry,scratch test and transwell cell invasion assay,respectively. After treament with different concentrations of wogonin for 48 h,the protein levels of β-catenin,C-myc and Cyclin D1 were detected by Western blotting. Results： Wogonin within the range of 20 - 200 mol / L could inhibit the proliferation of SGC7901,BGC-823 and MKN-45 cells in a dose-and time-dependent manner. Compared with 0 μmol / L wogonin group,After treament with different concentrations of wogonin for 24 h and 48 h,SGC7901 cells had elevated apoptosis rate and decreased migration distance and number of penetrating membrane cell in the rest concentrations（ P〈0. 05）. The protein levels of β-catenin,C-myc and Cyclin D1 were lower in 20 - 200μmol /L wogonin groups than 0 μmol /L wogonin group except for the concentration of 20 μmol /L wogonin groups（ P〈0. 05）,and the effects in a dose-dependent manner. Conclusion： Wogonin has the ability to inhibit the proliferation of gastric cancer cells,and can induce apoptosis and inhibit cell migration and invasion,which may be related to the inhibition of Wnt / β-catenin signaling pathway activation.
作者
汪晓辉
卫莹芳
程红
WANG Xiao-hui WEI Ying-fang CHENG Hong(College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China)
出处
《中药材》
CAS
CSCD
北大核心
2016年第6期1372-1376,共5页
Journal of Chinese Medicinal Materials
基金
国家科技基础性工作专项重点项目(2007FY110600)
