摘要
为研究饲用金霉素对獭兔盲肠菌群和免疫功能的影响,采用聚合酶链式反应-变性梯度凝胶电泳(PCRDGGE)和实时荧光定量PCR(qPCR)技术分析獭兔盲肠菌群;酶联免疫吸附试验(ELISA)测定细胞因子;qPCR技术对TLR2、TLR4mRNA进行定量检测。试验分为2组:对照组饲喂基础日粮;金霉素组在基础日粮中添加金霉素50mg/kg。结果表明:1)PCR-DGGE图谱显示,与对照组比较,金霉素组盲肠菌群结构和多样性等没有显著变化(P>0.05);2)qPCR检测金霉素组普雷沃氏菌属数量是10.48(logcopy/g),对照组是10.10(logcopy/g),差异显著(P<0.05);3)金霉素组獭兔外周血细胞因子IL-2、IL-4、TNF-α的浓度显著升高(P<0.05),脾脏和肝脏指数均降低;4)回肠的TLR2、TLR4mRNA相对表达量和肝脏的TLR2 mRNA相对表达量均高于对照组,但差异不显著(P>0.05)。在饲料中添加50mg/kg金霉素,对獭兔盲肠菌群结构和免疫功能均没有显著影响。
Experimental animals were randomly divided into two groups： Control group was fed basal diet without any antibiotics,Chlortetracycline group were fed basal diet added with 50 mg/kg chlortetracycline. Microflora changes in rex rabbit cecum were studied by PCR-DGGE and qPCR technologies. ELISA detection method was applied on cytokine determination, qPCR technology was used to analysis rex rabbits organizations for TLR2 and TLR4 mRNA. PCR-DGGE profile results showed chlortetracycline,the structure and diversity of cecum microflora were not significantly changed （P 〉 0. 05） compared with the control group, qPCR detection results showed that Bacteroides-Prevotella- Porphyromonas of the chlortetracycline and control group was 10. 48 and 10. 10 （log^copy/g ）, respectively. The chlortetracycline group ＇ s Bacteroides-Prevotella-Porphyromonas was greatly increased （ P 〈 0. 05）. Effect of chlortetracycline on immune function, peripheral blood cytokines concentrations IL-1, IL-2, IL-4, TNF-α were increased significantly （P〈0.05）. Spleen and liver indexes were decreased. TLR2 and TLR4 mRNA in ileum and TLR2 mRNA in liver relative expression level were higher than control group,but not significant （P〉0. 05）. The results suggested that adding of 50 mg/kg chlortetracycline in basal diet had no significant effect on the microflora in rex rabbits cecum and its immune function.
作者
文斌
邹芙沁
曾东
汪平
郭小林
倪学勤
傅祥超
刘宁
张凯
杜丹
徐昌文
WEN Bin ZOU Fu-qin ZENG Dong WANG Ping GUO Xiao-lin NI Xue-qin FU Xiang-chao LIU Ning ZHANG Kai DU Dan XU Chang-wen(Sichuan Academy of Grassland Science, Chengdu 611731, China Animal Micro-Ecology Institute/College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.)
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2016年第10期85-92,共8页
Journal of China Agricultural University
基金
四川省"十二五"农作物及畜禽育种攻关项目(2011NZ0099-4)
国家兔产业技术体系项目(CARS-44-A-4)
四川省科技厅2014年基本科研业务项目
