摘要
目的研究当归补血合剂对放射性肺炎模型小鼠血清转化生长因子(TGF-β1)、超氧化物歧化酶(SOD)的影响,以了解当归补血合剂对放射性肺炎的防治作用。方法将60只昆明小鼠随机分为4组:对照组、单纯给药组、单纯照射组、照射给药组;每组15只,建立急性放射性肺炎小鼠模型,以当归补血合剂灌胃给药,照射后21 d,检测TGF-β1以及SOD的含量变化;并制作小鼠肺组织切片行HE染色,观察小鼠肺组织病理改变。结果照射给药组血清TGF-β1浓度低于单纯照射组,照射给药组和单纯照射组TGF-β1的浓度均高于对照组和单纯给药组,差异有统计学意义(P<0.01);而照射给药组以及单纯照射组血清SOD的浓度低于对照组和单纯给药组,但照射给药组SOD浓度高于单纯照射组,差异均有统计学意义(P<0.01)。结论当归补血合剂可降低急性放射性肺炎小鼠的血清TGF-β1浓度,提高血清SOD的含量,在一定程度上减轻急性肺损伤,对放射性肺炎有一定的防护作用。
Objective To observe the influences of Danggui Buxue Decoction on transforming growth factor β1（ TGF- β1） and superoxide dismutase（ SOD） on radiation pneumonia mouse model,so as to reveal the impacts of Danggui Buxue Decoction on radiation- induced pneumonia. Methods 60 Kunming mice were randomly divided into four groups： blank control group,simple Danggui Buxue Decoction group,simple radiation group,radiation ＋ Danggui Buxue Decoction group. Each group contained15 mice. We constructed the acute radiation- induced pneumonia mouse model by whole- thorax irradiation,which were given Danggui Buxue Decoction. After 21 days irradiation,all groups of mice were collected to detect the level of TGF- β1 and SOD.Fixed with formalin,the lung tissue was stained with HE,we examined the pathological changes of lung tissue. Results Compared with the radiation group,the serum concentration of TGF- β1 in radiation ＋ Danggui Buxue Decoction group was lower than the simple radiation group,and the serum concentrations of TGF- β1 in both radiation ＋ Danggui Buxue Decoction group and simple radiation group were higher than the control group and simple Danggui Buxue Decoction group,with the differences statistically significant（ P 〈 0. 01）. However,the concentrations of SOD in radiation ＋ Danggui Buxue Decoction group and simple radiation group were lower than the control group and simple Danggui Buxue Decoction group,while the concentration of SOD was higher in the radiation ＋ Danggui Buxue Decoction group than in the simple radiation group,with the differences statistically significant（ P 〈 0. 01）. Conclusion Danggui Buxue Decoction can decrease the serum concentration of TGF- β1 in acute radiation- induced pneumonia mice and increase the serum concentration of SOD,to some extent,it could alleviate the acute radiation- induced lung injury.
作者
刘利胜
刘美芹
龚继勇
陈海生
刘敏
LIU Li-sheng LIU Mei-qin GONG Ji-yong CHEN Hai-sheng LIU Min(Clinical Laboratory,Shandong Cancer Hospital Affiliated to Shandong University, Ji ＇ nan, Shandong 250117, China)
出处
《中国卫生检验杂志》
CAS
2016年第20期2920-2921,2924,共3页
Chinese Journal of Health Laboratory Technology
基金
山东省中医药科技发展计划普通项目(2013-211)
关键词
放射性肺炎
转化生长因子Β1
超氧化物歧化酶
当归
Radiation pneumonitis
Transforming growth factor β1
Superoxide dismutase
Chinese Angelica
