摘要
中国是乙型肝炎(乙肝)的高发国家,抗病毒治疗药物耐药突变的早期发现对于接受抗病毒治疗的乙肝患者至关重要。理想的临床检测方法应具有灵敏度高、特异性高、重复性好、检测结果准确、操作简单、通量高、价格适中以及能定量检测等特点,并可检出样本中的多种混合突变。本文根据上述临床需求,就近年来出现的一些乙肝耐药突变检测技术进行讨论,并介绍一种基于基质辅助激光解吸电离飞行时间质谱法的新检测技术。
China is a country with high incidence of hepatitis B （HBV）. The early detection of antiviral drug resistance mutations in patients with hepatitis B is essential for the treatment of hepatitis B patients. Theoretically, the ideal clinical detection method should be with high sensitivity and high specificity, good repeatability, accurate detection results and also has the advantages of simple operation, high flux, moderate price, available quantitative detection, and can detect a variety of mixed mutations in samples. In this paper, based on the above clinical requirements, the authors have discussed the detection technology of hepatitis B drug-resistance mutations in recent years, and introduced a new detection technology based on matrix-assisted laser desorption/ionization time- of -flight mass spectrometry（MALDI-TOF MS）.
作者
张树永
陈琛
马庆伟
曲芬
ZHANG Shu-yong CHEN Chen MA Qing-wei Qu Fen(Clinical Laboratory Center, 302 Military Hospital of China, Beijing 100039, China)
出处
《传染病信息》
2016年第5期311-314,共4页
Infectious Disease Information
基金
首都市民健康培育课题(Z151100003915151)
