摘要
目的探讨新风胶囊(XFC)改善干燥综合征(SS)患者高凝状态与miR-155/细胞因子信号传送阻抑物(SOCS1)/核因子κB(NF-κB)信号转导通路的关系。方法将66例SS患者随机分为XFC治疗组和硫酸羟氯喹(HCQ)治疗对照组各33例,分别予XFC及HCQ治疗。另选20名健康者作为正常对照组。全自动凝血仪测定凝血酶原时间(PT)、活化部分凝血酶原时间(APTT)、纤维蛋白原(FIG)、凝血酶时间(TT)、D-二聚体(D-D);ELISA检测外周血血清白细胞介素1β(IL-1β)、IL-4、IL-10、肿瘤坏死因子α(TNF-α)、P50、P65、NF-κB抑制蛋白α(IκBα)水平;同时采用实时荧光定量PCR检测p65、p50、IκBαmRNA水平;一步法荧光定量PCR反应测定miR-155含量;Western blot法检测P65、P50、SOCS1蛋白水平;魏氏法测定血沉(ESR);全自动生化分析仪测定超敏C反应蛋白(hs-CRP)。结果与NC组比较,SS患者凝血参数D-D、FIB明显升高;SS患者外周血miR-155、IL-1β、TNF-α、P50、P65、IκBα及炎症指标hs-CRP、ESR水平明显升高;IL-4、IL-10水平明显降低;凝血参数与细胞因子、NF-κB信号转导通路及炎症指标明显相关。药物干预后,两组凝血参数和相关实验室指标均有部分改善;与对照组比较,XFC组有效率显著高于HCQ组,且在降低FIB、D-D、miR-155、TNF-α、IL-1β、P50、P65、ESR、hs-CRP水平,增加SOCS1,IL-4、IL-10水平方面明显优于HCQ组。结论 XFC能有效降低SS患者血液高凝状态,其机制与抑制miR-155/SOCS1/NF-κB信号通路有关。
Objective To explore the relationship between Xinfeng Capsule（ XFC） improving the hypercoagulative state in patients with Sjgren＇s syndrome（ SS） and miR-155 / suppressor of cytokine signaling 1（ SOCS1） / nuclear factor κB（ NF-κB）signaling pathway. Methods Sixty-six SS patients were randomly divided into XFC-treated group and hydroxychloroquine（ HCQ）-treated control group（ n = 33 per group）,which were respectively treated with XFC and HCQ. In addition,20 healthy volunteers were enrolled as a normal control group. The levels of prothrombin time（ PT）,activated partial thromboplastin time（ APTT）,fibrinogen（ FIG）,thrombin time（ TT） and D-dimer（ D-D） were detected using automatic coagulation analyzer.Interleukin-1β（ IL-1β）,IL-4,IL-10,tumor necrosis factor-α（ TNF-α）,P50,P65,inhibitor of NF-κB α（ IκBα） were tested using ELISA. Meanwhile,the mRNA expressions of p50,p65 and IκBα were determined using quantitative real-time PCR,and the level of microRNA-155（ miR-155） was examined by one-step fluorescence quantitative PCR. The protein levels of P50,P65 and SOCS1 were detected using Western blotting. Erythrocyte sedimentation rate（ ESR） was evaluated by Westergren method. Hypersensitive C-reactive protein（ hs-CRP） was detected using automatic biochemical analyzer. Results Comparedwith the normal control group,the levels of D-D and FIB significantly increased in SS group; simultaneously,the serum levels of miR-155,IL-1β,TNF-α,P50,P65,IκBα,hs-CRP,ESR were significantly elevated in SS patients,while IL-4 and IL-10 were significantly reduced. Spearman correlation analysis showed that the coagulation parameters were remarkably correlated with cytokines,NF-κB and activity indexes. In the two treated groups, coagulation parameters and related indexes were demonstrated having some improvement,especially in the XFC group,which had a much higher efficiency,and better outcomes in reducing the levels of FIB,D-D,miR-155,TNF-α,IL-1β,P50,P65,ESR and
作者
朱福兵
刘健
王桂珍
方利
章平衡
谈冰
ZHU Fubing LIU Jian WANG Guizhen FANG Li ZHANG Pingheng TAN Bing(Anhui University of Chinese Medicine, Hefei 230038 Department of Rheumatism Immunity, First Affiliated Hospital, Anhui University of Chinese Medicine, Hefei 230031, China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2016年第10期1366-1371,共6页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81173211/H2708)
国家中医药重点学科中医痹病学建设项目(国中医药发[2009]30号)
国家临床重点专科中医风湿病科建设项目(财社[2013]239号)
国家科技支撑计划课题(2012BA126B02)
安徽省重点实验室建设项目(1306c083035)
安徽省科技攻关项目(1301042211)
