摘要
目的:验证电针结合天麻素对局灶性脑缺血大鼠海马CA3区生长相关蛋白-43(Growth-associated protein,GAP-43)和突触素(Synaptophysin,SYN)表达是否有协同增效作用。方法:SD大鼠40只随机分为假手术组、模型组、电针组、天麻素组和电针结合天麻素组。采用线栓法阻塞大鼠右侧大脑中动脉复制局灶性脑缺血模型。造模成功后,按分组分别及同时给予电针刺激左侧曲池与合谷穴30min,天麻素注射液10mg/kg腹腔注射,1次/d,连续治疗14d。免疫组织化学SABC法检测海马GAP-43和SYN的表达。结果:与假手术组比较,模型组大鼠缺血侧海马CA3区GAP-43阳性神经元数量增多(P<0.05),SYN阳性神经元数量减少(P<0.05);与模型组比较,电针组、天麻素组和电针结合天麻素组大鼠缺血侧海马CA3区GAP-43和SYN阳性神经元数量均显著增多(P<0.05);电针结合天麻素组GAP-43和SYN阳性神经元数量均较电针组、天麻素组增多(均P<0.05)。结论:电针结合天麻素对缺血侧海马CA3区GAP-43和SYN的表达具有协同增效作用,可更好地促进缺血损伤神经元的再生修复。
Objective：To validate whether there is synergy effect of electroacupuncture（EA）combined with gastrodin on the expression of（Growth-associated protein,GAP-43）and（Synaptophysin,SYN）in hippocampal CA3 in focal cerebral ischemia（FCI）rats.Methods：Forty SD rats were randomly divided into sham operation,model,EA,gastrodin and EA＋ gastrodin groups.FCI rats were induced by middle cerebral artery occlusion with thread embolus.EA was applied to left side＂Quchi＂（LI 11）and＂Hegu＂（LI 4）after MCAO,gastrodin at 10mg/kg was given to rats by intraperitoneal.The expressions of GAP-43 and SYN in hippocampal CA3 detected by immunohistochemical.Results：Compared with sham operation group,the number of GAP-43 positive neurons increased（P〈0.05）,the number of SYN positive neurons decreased（P〈0.05）.Compared with model group,the number of GAP-43 and SYN positive neurons increased（P〈0.05）,expression levels in the EA＋gastrodin group increased significantly than the EA or gastrodin group（P〈0.05）.Conclusion：EA combined with gastrodin have synergistic effect on the expression of GAP-43 and SYN in the ischemic hippocampus CA3,can promote the regeneration and repair of injured neurons.
作者
陈鹏
王圣宽
张大雁
吴思苇
吴锋
李怀斌
CHEN Peng WANG Sbengkuan ZHANG Dayan et al(Department of Clinical Medicine, Class of 2013, Wannan Medical College , Wuhu City ,Anhui Province 241002)
出处
《医学理论与实践》
2016年第20期3441-3443,共3页
The Journal of Medical Theory and Practice
基金
国家级及安徽省大学生创新训练计划项目(201410368017,AH201410368017)
皖南医学院重点科研项目培育基金(WK2014Z07)
安徽省高校省级自然科学项目(WK2015A227)
