摘要
目的研究子宫内膜异位症对Wistar大鼠胚胎种植期子宫内膜整合素α_vβ_3、血管内皮生长因子(VEGF)基因的表达及雌、孕激素水平与不孕的关系。方法采用同种异体移植方法建立Wistar大鼠子宫直肠陷凹、腹壁外子宫内膜异位症动物模型36只,空白对照20只,建模成功后投放雄鼠,受孕4.5 d后麻醉采血,取模型大鼠在位子宫内膜组织,进行透射电镜观察,ELISA检测血清中雌激素、孕激素的含量,同时运用荧光定量PCR技术,定量分析整合素α_vβ_3以及VEGF基因在其子宫内膜中的表达。结果早孕各组大鼠子宫内膜α_vβ_3、VEGF基因的表达水平均高于模型组和空白未孕组;早孕能提高模型组α_vβ_3、VEGF基因的表达但均低于空白受孕组(P<0.05),且子宫直肠凹陷子宫内膜异位症模型大鼠的表达水平较腹壁外子宫内膜异位症模型大鼠低(P<0.05);子宫直肠凹陷子宫内膜异位症模型大鼠血清雌激素水平低于空白受孕组(P<0.05),但孕激素较空白受孕组高(P<0.05)。结论不同程度的子宫内膜异位症均能降低Wistar大鼠窗口期整合素α_vβ_3、VEGF基因的表达,使其达不到正常胚胎种植期的水平是导致不孕的重要原因。
Objective To investigate the expression of integrin αvβ3 and VEGF gene in endometriosis model rats 4.5 days after mating and detect the content of estrogen and progesterone, which can explains its relationship with infertility. Methods Allografts uterus were transplanted into 38 female wistar rats to establish the uterine rectal pouch endometriosis model, another 20 Wistar rats were used as the comparison group. Male rats were permitted to approach the female models 8 weeks after transplantation, The eutopic and ectopic endometrium were collected 4.5 days after mating. Transmission electron microscopy was used for histological analysis, and the expression of integrinαvβ3 receptor and VEGF gene in endometrial was tested by RT-PCR method. The serum estrogen and progesterone levels were detect by ELISA. Results The expression levels of integrinαvβ3 and VEGF in uterine rectal pouch endometriosis model rats were significantly lower than those of the abdominal wall endometriosis model rats （P〈0.05）. And conception could both improve the expression of the two gene but still could not retain to the normal expression level （P〈0.05）. Meanwhile, the content of serum estrogen and progesterone in the abdominal wall endometriosis model rats were slightly lower than the normal level（P〉0.05）. The content of serum estrogen level in uterine rectal pouch endometriosis model rats was significantly lower than the normal level （P〈0.05）, but the content of progesterone was significantly higher than the normal level（P〈0.05）. Conclusion The expression of integrinαvβ3 and VEGF gene and the contents of serum estrogen and progesterone were affected by endometriosis that could not return to the normal expression level and might lead to infertility.
出处
《热带医学杂志》
CAS
2016年第10期1227-1230,1234,共5页
Journal of Tropical Medicine
基金
广东省科技基础条件建设项目(2012B060300015)
