摘要
背景:脂肪组织来源丰富,不同部位脂肪组织分离培养的脂肪干细胞在体外生物学特性上是否存在差异,目前相关研究较少。目的:观察肾脂肪囊来源与腹股沟来源脂肪干细胞的体外增殖和成软骨诱导分化能力。方法:收集大鼠肾脂肪囊来源与腹股沟区来源组织进行脂肪干细胞培养与鉴定,MTT法检测两组细胞的体外增殖能力。取第3代细胞进行软骨定向诱导2周,免疫荧光检测诱导后细胞CollagenⅡ表达,RT-PCR检测两组细胞诱导后Aggrecan、CollagenⅡm RNA表达水平。结果与结论:(1)肾脂肪囊来源与腹股沟区来源脂肪干细胞经原代与传代培养,形态基本一致;(2)经流式细胞仪检测,不同来源脂肪干细胞的CD44阳性率均达到95%以上;(3)两组细胞的体外生长曲线均呈"S"型;(4)诱导后两组细胞均表现出CollagenⅡ阳性表达;(5)两组细胞的Aggrecan、CollagenⅡmRNA表达水平比较差异无显著性意义(P>0.05);(6)结果显示,肾脂肪囊来源与腹股沟区来源脂肪干细胞均在体外生长稳定,迅速增殖,经定向诱导向软骨细胞发生分化,二者无明显区别。
BACKGROUND： Adipose-derived stem cells have different sources, but it is unclear whether these cells from different sources have difference in their biological properties. OBJECTIVE： To detect the in vitro proliferation and chondrogenic differentiation of adipose-derived stem cells derived from renal fat capsule and groin. METHODS： Adipose-derived stem cells from renal fat capsule and groin of rats were isolated, cultured and identified. MTT assay was used to detect in vitro proliferation ability of these cells. Passage 3 cells were under chondrogenic induction for 2 weeks. After induction, the expression of type II collagen was observed by immunofluorescence detection, and RT-PCR was employed to detect the expression levels of Aggrecan and type II collagen mR NA in the two groups. RESULTS AND CONCLUSION： After primary culture and passage, adipose-derived stem cells from the renal fat capsule and groin of rats exhibited similar morphology, and over 95% of cells expressed CD44 in the two groups. Adipose-derived stem cells from two sources showed an S-shaped growth curve in vitro and were positive for type II collagen. After RT-PCR detection, the expression levels of Aggrecan and type II collagen mR NA had no difference in adipose-derived stem cells from renal fat capsule and groin（P〈0.05）. Experimental results show that adipose-derived stem cells from both renal fat capsule and groin exhibit stable growth, rapid proliferation and chondrogenic differentiation under orient induction in vitro, indicating there is no difference between these cells from two sources.
出处
《中国组织工程研究》
CAS
北大核心
2016年第41期6197-6202,共6页
Chinese Journal of Tissue Engineering Research
