摘要
目的制备高、中、低分子质量的3种葡萄糖聚合物腹膜透析液,利用新西兰兔腹膜透析模型,考察其对超滤、小分子清除以及腹膜生物相容性影响的异同。方法药效实验选择健康的新西兰白兔,完全随机分为3组,即高、中、低分子质量葡萄糖聚合物透析组,每组8只。于透析240 min结束时,收集血液和透析流出液样本。分别检测血浆及透析流出液中肌酐、尿素氮的浓度,并测量透析流出液总体积。生物相容性试验中,考察中分子质量葡萄糖聚合物腹膜透析液连续给药3天对新西兰兔腹膜的组织形态学影响。结果本研究通过控制合成条件,分别制备了高、中、低3种分子质量的葡萄糖聚合物,并制备成腹膜透析液。结果发现3种相对分子质量的葡萄糖聚合物腹膜透析液在超滤量(F=8.875,P=0.002)、尿素氮清除率(F=13.090,P<0.001)和肌酐清除率(F=6.429,P=0.007)的差异均具有统计学意义。其中,中分子质量葡萄糖聚合物腹膜透析液具有更好的超滤作用[与低分子质量相比:(63±9)ml比(45±8)ml,q=5.910,P=0.001;与高分子质量相比(63±9)ml比(52±8)ml,q=3.612,P=0.047],而且对小分子溶质尿素氮[与低分子质量相比:(0.485±0.051)ml/min比(0.372±0.046)ml/min,q=6.562,P<0.001;与高分子质量相比:(0.485±0.051)ml/min比(0.383±0.049)ml/min,q=5.923,P=0.001]和肌酐[与低分子质量相比:(0.536±0.064)ml/min比(0.436±0.057)ml/min,q=4.887,P=0.006;与高分子质量相比:(0.536±0.064)ml/min比(0.462±0.052)ml/min,q=3.616,P=0.046]的清除作用也最强。腹膜刺激性研究证实中分子质量葡萄糖聚合物腹膜透析液对腹膜组织形态学无影响。结论中分子质量葡萄糖聚合物腹膜透析液具有更好的治疗作用和良好的腹膜生物相容性。
Objectives Three different molecular weights of glucose polymers, namely large, medium,and small were prepared. Using New Zealand rabbit model for peritoneal dialysis（PD）, the effect of those glucose polymers peritoneal dialysis fluids on the net ultrafiltration and the capacity of creatinine and urea nitrogen removal and its effect on peritoneal biocompatibility were evaluated. Methods In pharmacological experiment, healthy New Zealand white rabbits were randomly divided into three groups, i.e. low, middle and high molecular glucose polymer PD groups（8 animals in each group）. After dwelling of the dialysate for 240 min, blood and effluent fluids were collected, and the concentration of creatinine and urea nitrogen in plasma and effluent fluids were measured. Furthermore, the effect of middle molecular weight glucose polymer for three consecutive days on peritoneal histomorphology was evaluated for biocompatibility study. Results Three different molecular weights of glucose polymers were developed by controlling synthesis conditions and the glucose polymer peritoneal dialysis fluids were prepared. In vivo studies showed that there were statistically differences in net ultrafiltration（F=8.875, P=0.002）, urea nitrogen clearance（F=13.090, P〈0.001）, and creatinine clearance（F=6.429, P=0.007） among the three groups. In addition, the middle molecular had a higher ultrafiltration efficiency（63±9 ml vs. 45±8 ml, q=5.910, P=0.001, compared to low moelcular; 63±9 ml vs.52 ± 8 ml, q=3.612, P=0.047, compared to high molecular） and the better clearance of urea nitrogen（0.485 ±0.051 ml/min vs. 0.372 ± 0.046 ml/min, q=6.562, P〈0.001, compared to low molecular; 0.485 ± 0.051 ml/min vs. 0.383±0.049 ml/min, q=5.923, P=0.001, compared to high molecular） and creatinine（0.536±0.064 ml/minvs. 0.436±0.057 ml/min, q=4.887, P=0.006, compared to low molecular; 0.536±0.064 ml/min vs. 0.462±0.052ml/min, q=3.616, P=0.046, compared to high molecular）. Similarly, peritoneal irritation studies con
出处
《中国血液净化》
2016年第10期559-562,共4页
Chinese Journal of Blood Purification
关键词
葡萄糖聚合物腹膜透析液
超滤量
肌酐清除率
尿素氮清除率
Glucose polymer peritoneal dialysis fluid
Ultrafiltration
Urea nitrogen Clearance
Creatinine clearance
