摘要
目的确定吸烟对甲状腺相关眼病(TAO)患者眶脂肪分化的影响。设计回顾性病例系列。研究对象2012年6-12月在上海长征医院眼科行眶减压术的TAO患者40例,男性27例,女性13例。方法 40例TAO患者中,21例吸烟、8例曾吸烟但已戒烟,5例被动吸烟,6例非吸烟。运用RT-PCR检测所有患者眶脂肪CCAAT/增强结合蛋白(C/EBP)和过氧化物酶体增生物激活受体(PPARγ)mRNA表达量。用△Ct法进行其各基因表达的相对定量。多组间的总体比较采用单因素方差分析,随后用SNK-q检验进行两两比较。主要指标C/EBP和PPARγmRNA表达量。结果吸烟组患者的C/EBP(12.04±4.26)和PPARγmRNA表达量(4.82±1.41)明显高于非吸烟组(C/EBP:2.32±0.92;PPARγ:1.37±0.25)(P<0.001)。同时,曾吸烟组和被动吸烟组的C/EBP(4.29±1.26,5.76±1.03)和PPARγmRNA表达量(2.91±1.23,3.22±0.50)对比非吸烟组的差异也具有统计学意义(曾吸烟组C/EBP P=0.039,PPARγP=0.047;被动吸烟组C/EBP P=0.002,PPARγP=0.002)。吸烟指数≥100年支的TAO患者眶脂肪C/EBP(14.28±3.61)和PPARγmRNA(5.65±1.02)高于吸烟指数<100年支的患者(C/EBP:8.42±2.30;PPARγ:3.48±0.74)(P均<0.01)。结论眶脂肪C/EBP和PPARγmRNA的表达量与吸烟史有关。
Objective To determine whether smoking increases adipogenesis of the orbital fatty tissue in patients with thyriod associated ophthalmopathy（TAO）. Design Retrospective case series. Participants 40 patients （21 smokers, 8 ex-smokers, 5 passive smokers and 6 nonsmokers） were enrolled. Methods The expression levels of CCAAT/enhancer binging protein（C/EBP） and peroxisome proliferciter-activated receptor γ（PPARγ） of orbital fatty tissue were measured using real-time PCR. Main Outcome Measures C/EBP and PPARγ mRNA expressions. Results Orbital C/EBP and PPARγ mRNA expressions significantly increased in patients with history of smoking （C/EBP： smoker 12.04±4.26 vs non-smoker 2.32±0.92; PPARγ： smoker 4.82±1.41 vs non-smoker 1.37±0.25） （P〈0.001）, and their expression of ex-smokers and passive smokers were higher than nonsmokers （C/EBP： 4.29±1.26, 5.76±1.03; PPARγ： 2.91±1.23, 3.22±0.50; all P〈0.05）. Additionally, we found that the expressions of C/EBP （14.28±3.61） and PPARγ mRNA （5.65±1.02） in orbital adipose tissues from TAO patients with higher smoking index（≥100 cigarettes/day years） increased compared with those with lower smoking index（〈100 cigarettes/day years）（ C/EBP 8.42±2.30;PPARγ 3.48±0.74）（P〈0.01）. Conclusion Our study suggests that smoking at presentation for TAO increases the levels of C/EBP and PPARγ. （Ophthalmol CHN, 2016, 25： 334-338）
作者
周哲
高玉
孙琰
景明
胡佳丽
ZHOU Zhe GAO Yu SUN Yan JING Ming HU Jia-li(Department of Ophthalmology, No. 411 Hospital of PLA, Shanghai 200081, China)
出处
《眼科》
CAS
CSCD
北大核心
2016年第5期334-338,共5页
Ophthalmology in China
关键词
吸烟
甲状腺相关眼病
CCAAT/增强结合蛋白
过氧化物酶体增生物激活受体
smoking
thyriod-associated ophthalmopathy
CCAAT/enhancer binging protein（C/EBP）
peroxisome proliferciter-ac tivated receptor γ（PPARγ）
