摘要
目的:探讨早期氢水和有氧运动干预对心肌梗死大鼠左心室β_3-AR及其下游分子e NOS蛋白表达的影响。方法:雄性SD大鼠随机分为假手术组(S)、心肌梗死组(MI)、有氧运动+心梗组(EM)、氢水+心梗组(HM)和氢水+有氧运动+心梗组(HEM)。HM和HEM组大鼠均给予早期氢水灌胃4 m L,灌胃时间5 d/周×3周。EM和HEM组大鼠均进行早期3周递增式跑台运动。3周氢水及运动干预后MI,EM,HM和HEM组大鼠均结扎心脏左冠状动脉前降支,建立MI模型。S组大鼠实施假手术。5组大鼠术后45min测定血流动力学指标判定心功能变化。心脏Masson染色测定胶原容积百分比(CVF)。采用荧光免疫组化法检测左心室β_3-AR蛋白表达。采用western blot法检测β_3-AR及下游e NOS蛋白水平。结果:MI后心脏CVF值显著升高(P<0.01),心功能显著降低,同时左心室β_3-AR蛋白表达有增加趋势。与MI组比较,EM、HM和HEM组CVF(P<0.01)均显著降低,心功能均得到提升。EM、HM和HEM组均可见左心室β_3-AR阳性染色,同时EM和HM组β_3-AR和e NOS蛋白表达均较MI组显著增加(均P<0.05),HEM组β_3-AR和e NOS表达较MI组有增加趋势。且左心室β_3-AR与e NOS蛋白表达呈显著正相关(R=0.47,P<0.01)。结论:心梗之前进行3周氢水和运动干预可减轻心肌纤维化程度,提升心梗大鼠心功能,增加左心室β_3-AR表达,上调其下游e NOS蛋白表达。氢水联合运动干预对β_3-AR影响并不及单一干预效果。而3种干预方式对心功能提升并无差异。
Objectives： To discuss the effect of hydrogen-rich water and exercise prior to myocardial infarction onβ_3-AR and e NOS of left ventricular in rats. Methods： Sixty male Sprague-Dawley rats were assigned to five groups： sham group（ S）,myocardial infarction group（ MI）,aerobic exercise ＋ myocardial infarction group（ EM）,hydrogen-rich water ＋ myocardial infarction group（ HM）,and aerobic exercise ＋ hydrogen-rich water＋ myocardial infarction group（ HEM）. HM and HEM groups were suffered intragastric administration of 4 m L hydrogen-water,5 d / week,3 weeks in total. After 3 weeks,MI was induced by ligation of the left anterior descending coronary artery in all groups except S,S group was operated with sham operation. The hemodynamic parameters were measured to detect cardiac function 45 min after operation. The CVF was tested by Masson staining.Cardiac β_3-AR was detected by IFC and WB,and cardiac e NOS expression was detected by WB. Results： After MI,CVF increased（ P 0. 01）,cardiac function decreased,cardiac β_3-AR protein expression had an increasing trend. Compared with MI group,CVF of EM,HM and HEM groups significantly reduced（ P 0. 01）,cardiac function was improved,and cardiac β_3-AR was positively stained. Compared with MI group,β_3-AR and e NOS expressions of EM and HM were higher（ P 0. 05）,and it had higher trend of HEM. β_3-AR expression was positively correlated with e NOS protein expression（ r = 0. 47,P 0. 01）. Conclusions： Hydrogen-rich water and exercise prior to myocardial infarction can reduce myocardial interstitial fibrosis,promote cardiac function,and increase cardiac β_3-AR and eN OS expression. The effect of hydrogen-rich water or exercise prior to myocardial infarction on cardiac β_3-AR is better than combination intervention of hydrogen-rich water and exercise,and there is no difference of improving cardiac function among three interventions.
出处
《北京体育大学学报》
CSSCI
北大核心
2016年第9期63-69,共7页
Journal of Beijing Sport University
基金
国家自然科学基金资助项目(编号:81360297
31371199)
