摘要
目的:LCT制片法筛选肺腺癌组织的Ki67、EGFR基因蛋白表达效果分析。方法:肺部病变患者进行CT引导下经胸壁肺内肿物穿刺通过中采用LCT制片选择488例典型病例,取组织条进行镜下观察筛选肺腺癌,同时采用免疫组织化学法(IHC)Ki67、EGFR基因蛋白检测,以30条正常组织标本为对照。结果:LCT制片法细胞学检测选出肺腺癌88条,其中进一步组织学检测发现仅有85条为肺腺癌,占比(96.6%),无显著性差异(P>0.05);85条肺腺癌组织条中,Ki67基因蛋白和正常组织中的蛋白增殖活性高比率分别为56.47%、6.67%,具有明显差异(P<0.01);而EGFR蛋白增殖活性高比率分别为68.24%、0.0%,差异性显著(P<0.01);Ki67、EGFR表达与患者的肿瘤大小、组织学分型均无相关性(P>0.05),而与肿瘤的分化程度、浸润深度有关(P<0.01)。结论:LCT制片法筛选肺腺癌组织的Ki67、EGFR基因蛋白表达明显增高,有助于肺腺癌诊断。
Objective： To screen the expression of Ki67 and EGFR protein in lung adenocarcinoma by LCT.Methods： 488 patients with pulmonary lesions underwent CT-guided percutaneous transluminal puncture of the lungs were selected for LCT. The tissue sections were used to observe the lung adenocarcinoma. Immunohistochemistry（ IHC） Ki67 and EGFR protein were detected in 30 normal tissue samples. Results： There were 88 lung adenocarcinomas detected by LCT cytology. Only 85 lung adenocarcinomas were detected by histological examination（ 96. 6%）. There was no significant difference（ P〉0. 05）. The positive rates of Ki67 protein and normal tissue proliferative activity were 56. 47% and 6. 67%,respectively（ P〈0. 01）,and the high rate of EGFR protein proliferative activity was 68. 24% in 85 lung adenocarcinoma tissues,（ P〈0. 01）. The expression of Ki67 and EGFR had no correlation with tumor size and histological type（ P〉0. 05）,but was correlated with tumor differentiation and depth of invasion（ P〈0. 01））. Conclusion： LCT production method of screening of lung adenocarcinoma tissue including Ki67 and EGFR gene protein expression increased significantly,which facilitates diagnosis lung adenocarcinoma.
出处
《湖北职业技术学院学报》
2016年第3期109-112,共4页
Journal of Hubei Polytechnic Institute
