摘要
为研究栉孔扇贝消化盲囊和鳃丝对石油烃的富集排除规律及氧化损伤效应,在实验室条件下将栉孔扇贝在各浓度石油烃的海水中分别进行染毒和排除实验各15d,测定消化盲囊和鳃丝的石油烃蓄积量、石油烃蓄积排除速度、生物浓缩系数、溶菌酶活性和脂质过氧化水平等指标。结果表明,栉孔扇贝两组织在蓄积期间石油烃含量随时间呈现增加趋势,在9d之后达到蓄积量的平衡,浓度越高,生物浓缩系数BCF越小;0.1mg·L^(-1)和0.3mg·L^(-1)石油烃处理组两组织蓄积和排除速度随石油烃浓度升高而降低,在排除期间最后阶段都能将石油烃完全排除,蓄积期间溶菌酶活力和脂质过氧化(LPO)水平随时间不断上升,排除期间均能恢复到正常水平,而1.0mg·L^(-1)和3.0mg·L^(-1)处理组两组织蓄积期间的溶菌酶活力则呈现明显被抑制的现象,LPO水平明显升高,且在排除期间均不能恢复到对照组水平。结果证实,栉孔扇贝对0.3mg·L^(-1)及以下低浓度石油烃具有较好的应对机制和耐受性,而在1.0mg·L^(-1)浓度以上石油烃处理组其调控能力受到了明显的影响,机体处于较强的氧化损伤状态。本实验各指标均能体现不同浓度石油烃对栉孔扇贝组织的影响规律,且相互之间存在着非常相似的变化和紧密联系,能准确反映机体的解毒调控机制和机体氧化损伤效应规律。
Accumulation, Elimination law and Oxidative Damage Effect under Petroleum Hydrocarbon Exposure for Scallop Chlamys farreri were studied, The scallop Chlarnys farreri were set in different concentrations petroleum hydrocarbon （0.1 mg· L-1, 0.3 mg. L 1, 1.0 mg·L-1 and 3.0 mg· L-1） exposures and then were separately for 15 days, and then were transferred to fresh seawater not include petroleum hydrocarbon for 15 days. The petroleum hydrocarbon contents, accumulation and elimination rates, bio-concentration factor （BCF）, lysozyme activity and lipid peroxidation （LPO） level of diges- tive gland and gills were measured soon. The results showed that the petroleum hydrocarbon contents increased with the time, and then maintained the balance after 9 days, the BCF were lower if the concentrations of the petroleum hydrocarbon were higher. The accumulation and elimination rates of two tissues decreased if the concentrations of the petroleum hydrocarbon were higher in 0.1 mg · L-1 and 0.3 mg ·L-1 groups, the petroleum hydrocarbon could be eliminated completely at last in elimination period, the lysozyme activities and LPO levels increased with time in accumulation period and could recover to controls at last. The lysozyme activities of two tissues were restrained significantly in 1.0 mg ·L-1and 3.0 mg · L-1 groups, the LPO level increased significantly, and they can not recover to controls levels. The results indicated that the scallop Chlarnys farreri have the well coping mechanism and tolerability to petroleum hydrocarbon less than 0.3 mg · L-1 coficentrations, but it＇s control ability was restrained by petroleum hydrocarbon greater than 1.0 mg · L-1 concentrations, the organisms were in oxidative damage seriously. The biomarkers of this experiment all could reflect the influence laws of different concentration petroleum hydrocarbon on scallop Chlamys farreri, and there was a very similar change and close relationship with each other, so it was accurate to reflect the detoxification mechanism and the
作者
任加云
李学平
苗晶晶
REN Jiayun LI Xueping and MIAO Jingjing(Department of Urban and Environment, Binzhou University of China, Binzhou 256600, China The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China)
出处
《海洋湖沼通报》
CSCD
北大核心
2016年第4期111-119,共9页
Transactions of Oceanology and Limnology
基金
山东省自然科学基金青年项目(ZR2014CQ048)
关键词
石油烃
栉孔扇贝
富集
排除
溶菌酶
LPO
petroleum hydrocarbon
Chlamys farreri
accumulation
elimination
lysozyme
lipid peroxidation
