摘要
目的:研究经腹肾脏去神经联用氯沙坦对高血压大鼠心脏肥厚的作用机制。方法:以高血压心脏肥厚的大鼠为研究对象,干预治疗4周,心脏超声探测左室壁厚度、反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)检测肥厚基因的表达、测定左室重量指数和组织病理切片苏木精-伊红染色法(hematoxylin-eosin staining,HE)观察病理改变。结果:12周龄的自发性高血压大鼠,左心室病理观察和超声探测显示心肌呈肥厚改变。干预治疗4周,病理观察发现,肾去神经治疗(renal denervation,RDN)和肾去神经联合氯沙坦(renal denervation combined with Losartan,R+L)治疗组病理性心肌肥厚有所改善,以后者更明显。左心室体重指数、局部心肌组织相对细胞表面积、相对肥厚基因的表达、左心室室壁厚度、炎症因子的表达与病理结果一致,比较有统计学差异。结论:肾脏去神经可抑制高血压心肌肥厚,联用氯沙坦更显著,其机制可能与减少心肌组织炎症因子的表达相关。
Objective To explore the effect and mechanism of renal denervation combined with Losartan on cardiac hypertrophy in spontaneous hypertensive rats. Methods Spontaneous hypertensive rats with cardiac hypertrophy underwent renal denervation combined with following 4 weeks drug treatments. Transthoracic two-dimensional guided M-mode echocardiography was performed. The expression of hypertrophy-related gene was detected. Left ventricularmass index was calculated and histological sectionsstained with hematoxylin and eosin （HE）. Results The characteristics of cardiac hypertrophy was observed in left ventricular tissues with HE staining inl2 weeks old spontaneous hypertensive rats.The thickness was increased in those ventricular （IVSs, IVSd, LVPWs and LVPWd）. After4 weeks after treatment, histological improvements were observed in both RDN and R＋L groups compared with sham group. The improvement was more obvious in R＋L group. Similar results were observed in histological sections, ventricular thicknesses （IVSs, IVsd, LVPWs and LVPWd）, related- hypertrophy genes （ANP and β-MHC） expression, left ventricular mass idex （LVMI） and the protein expression of inflammatory cytokines （TNF-α and IL-6）. Conculsion Renal denervation therapy can improve hypertensiveinduced cardiac hypertrophy in spontaneous hypertensive rats. The effect was more significant when combined with Losartan. The mechanism might be involved in inhibiting inflammatory eytokines.
作者
区德锦
林育辉
戴文军
伍金雷
谢文杰
黄炯华
Ou De-jin Lin Yu-hui Dai Wen-jun Wu Jin-lei Xie Wen-jie Huang Jiong-hua(Department of Laboratory Medicine, Department of cardiology,the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, Chin)
出处
《实用医学杂志》
CAS
北大核心
2016年第18期2978-2983,共6页
The Journal of Practical Medicine
关键词
高血压心脏肥厚
肾脏去神经
氯沙坦
联合治疗
作用机制
Hypertension-related cardiac hypertrophy
Renal denervation
Losartan
Combination therapy
Mechanism
