摘要
目的探讨YAP蛋白在临床不同分期、恶性程度不同的胃癌组织中的表达差异,及其同胃癌死亡率的影响。方法选择2013年8月至2015年1月湖北省江汉大学附属医院胃癌临床Ⅰ期组织块17例、Ⅱ期胃癌组织35例、Ⅲ期胃癌组织42例和Ⅳ期31例,以及相应的癌旁组织作为对照,采用免疫组化(IHC)和Western blot方法分别检测YAP蛋白表达水平,分析其与胃癌死亡率的相关性。结果胃癌组织中YAP表达水平显著高于癌旁组织(P=0.002 1),差异有统计学意义(P<0.05)。YAP在不同分期的胃癌病人中表达也具有差异性,且恶性程度越高的胃癌组织中,YAP表达量越多;YAP表达水平与临床Ⅲ期(P=0.020)和临床Ⅳ期(P=0.007)患者死亡率有显著性相关,YAP高表达的胃癌病人生存率显著低于YAP低表达的胃癌病人。结论胃癌的恶性程度与YAP的表达呈正相关,异常高表达YAP的胃癌病人一般伴随不良预后和高死亡率。
Objective To explore YAP（Yes associated protein） expression levels at different clinical stages tissues of gastric cancers,and itsassociation with the mortality of patients. Methods 17 cases of clinical stageⅠ, 35 cases of stageⅡ, 42 cases of stage Ⅲ and 31 cases of stage Ⅳof gastric cancer were selected for the study. YAP protein expression level was detected by immunohistochemistry（IHC） and Western blot. Results YAP expression in gastric cancer tissue were significantly higher than that of normal tissue adjacent to carcinoma（P=0.002 1）.The higher the malignant degree of gastric cancer tissues, the higher was the YAP expressing level. YAP protein expression level and clinical Ⅲ stage（P=0.020）and clinical Ⅳ stage（P=0.007） mortality in patients with significant correlation, the difference was statistically significant（P〈0.05）; The survival rate of gastric cancer patient with YAP high expression was significantly lower than that with YAP negative. Conclusion The malignant degree of gastric cancer was positively correlated with the expression level of YAP,abnormally high expression of YAP in gastric cancer patients usually associated with poor prognosis and with high mortality.
出处
《热带医学杂志》
CAS
2016年第9期1174-1176,1180,F0004,共5页
Journal of Tropical Medicine
