摘要
目的探究SPOPL蛋白（speckle—typePOZprotein—like）在胶质瘤组织中的表达及其临床意义。方法收集68例胶质瘤患者病例标本及瘤旁正常脑组织，其中低级别胶质瘤32例（whoI-Ⅱ），高级别胶质瘤36例（wHOⅢ-Ⅳ），用免疫组织化学法、蛋白质印迹法检测SPOPL蛋白表达，并分析临床指标与SPOPL表达量之间的关系。结果胶质瘤组织中SPOPL的表达低于瘤周组织（t=8．754，P〈O．05），高级别胶质瘤中SPOPL的表达低于低级别胶质瘤（t=13．552，P〈O．05）；SPOPL的表达与病理级别有关（X2=4．023，P〈O．05）；Cox回归模型提示肿瘤病理级别、SPOPL蛋白表达差异是胶质瘤患者预后的独立危险因素。结论SPOPL蛋白的表达与胶质瘤病理级别及患者预后呈负相关，其可能是反应胶质瘤生物学特征及患者预后的的一个新的分子标记物。
Objective To explore the expression of Speckle-type POZ protein-like （SPOPL）in human gioma tis- sues and its relationship with clinical pathological parameters and prognosis. Methods Immunohistochemical and west- ern blotting method were used to detect SPOPL expression in glioma tissues and tumor peripheral tissues in 68 cases of glioma patients including 32 cases of low grade gliomas （WHO I - II）, and 36 cases of high grade gliomas （WHO III - IV）. T test, A？ test, Kaplan-Meier method and the Cox regression model were used to analyze the relationship between the expression and clinical indicators. Results The expression of SPOPL was significantly lower in gliomas than in tumor pe- ripheral tissues （t=-8.754, P〈0.05）, the expression of SPOPL was lower in high pathological grade tissues than in low grade of glioma tissues （t=-13.552, P〈0.05）. SPOPL expression was associated with pathological grade （x2=4.023, P〈 0.05）. Cox regression model showed that the tumor pathological grade and different SPOPL protein expression were inde- pendent risk factors for the prognosis of patients with glioma. Conclusions SPOPL may be a biomarkers of human brain gliomas and can help to evaluate the prognosis of brain glioma.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2016年第8期469-472,共4页
Chinese Journal of Nervous and Mental Diseases
基金
广州市科技计划项目产学研协同创新重大专项(编号:2016-0402004)资助
