摘要
利用单交换法将gfp整合到鱼腥蓝细菌PCC 7120基因组上,构建ftsZ-gfp的翻译融合菌株,并观察了FtsZ蛋白的亚细胞定位。结果表明:通过同源重组,gfp与基因组中唯一完整的ftsZ融合,既保证了ftsZ基因的正常表达又能准确反映ftsZ蛋白的亚细胞定位,并且实验周期短、操作方便,是研究蛋白质亚细胞定位的一种简单有效的方法。
A ftsZ-gfp fusion strain was constructed to observe the subcellular localization of FtsZ, in which the ftsZ-gfp was integrated into genome in situ by single-crossover recombination. The results showed that this method guaranteed the normal expression of the gene and accurately reflected the subcellular localization of the protein. It is a simple and effective method for studying the subcellular localization of proteins.
作者
牛天彩
胡胜
陈雯莉
王莉
NIU Tiancai HU Sheng CHEN Wenli WANG Li(State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 130070 , China)
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2016年第6期30-36,共7页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31570048)
