摘要
以文心兰‘红狐狸’与‘百万金币’杂种胚为外植体,采用种胚→原球茎→完整植株→移栽的途径,探讨植物生长调节剂、有机添加物等因素对文心兰杂种胚萌发、原球茎分化、生根等各阶段的影响,建立文心兰杂种胚离体培养及植株再生技术。结果表明,适宜萌发的培养基为花宝+6-BA 0.5 mg·L^(-1)+NAA 0.1mg·L^(-1)+CM 150g·L^(-1)+AC 1.0g·L^(-1)+蔗糖20g·L^(-1),培养90~100d后相对萌发率为155.6%,原球茎基本不增殖,利于叶的分化;适宜分化的培养基为花宝+6-BA 0.1mg·L^(-1)+NAA 0.1mg·L^(-1)+马铃薯泥150g·L^(-1)+AC 1.0g·L^(-1)+蔗糖25g·L^(-1),分化率显著最高,为87.8%,添加马铃薯泥能显著提高分化率,促进芽苗的粗壮;将分化的芽苗在花宝+NAA 0.2mg·L^(-1)+马铃薯泥150g·L^(-1)+AC 1.5g·L^(-1)+蔗糖25g·L^(-1)培养基上培养40d后生根率为100%,且根系发达,植株健壮;生根苗炼苗后以水苔作为基质,移栽成活率达91.7%。
Embryos of the orchidhdyridcrossed between Beallara Marfitch Howards Dream and Oncidium Sweet Sugar Million Dollar were usedas explants in a cultivation experimentation to study the effects of various growth regulators and organic additives on its germination,protocorm differentiation,and rooting,from the formations of embryos and protocorms to the development and transplantation of the plants.Aculture medium consisting of Hyponex,6-BA 0.5mg·L^-1,NAA 0.1 mg·L^-1,CM 150g·L^-1,AC 1.0g·L^-1,and sugar 20g·L^-1provided an optimal relative embryo germination rate of 155.6% for the hybrid after 90-100 dof cultivation.In addition,the medium inhibited the protocorm proliferation,and thus,encouraged its differentiation subsequently.For the protocorm differentiation,a medium containing Hyponex,6-BA 0.1 mg·L^-1,NAA 0.1 mg·L^-1,mashed potato 150g·L^-1,AC 1.0g·L^-1,and sugar 25g·L^-1 rendered asignificantly higher rate of 87.8%thanother media tested.The addition of mashed potatoes in the medium significantly increased the differentiation ratepromoting the seedling growth.Vigorously growing plantlets were,then,transferred to a medium containing Hyponex,NAA 0.2mg·L^-1,mashed potato 150g·L^-1,AC 1.5g·L^-1,and sugar 25g·L^-1 for a complete and well-developed rooting on the healthy and well-developed plantlets with 40 dof cultivation.After an initial adaptation period,the survival rate of the transplanted seedling sreached 91.7% on peat moss.
出处
《福建农业学报》
CAS
北大核心
2016年第8期839-843,共5页
Fujian Journal of Agricultural Sciences
基金
福建省林业科学研究项目[闽林种站(2013)42号]
福建省农业科学院导师制青年科技创新基金项目(2013DQA-4)
关键词
文心兰
杂种胚
胚培养
原球茎
oncidium
hybrid embryo
embryo culture
protocorm
