摘要
目的：采用高效液相色谱法（HPLC）测定对叶百部根蜜炙前后对叶百部碱的含量。方法采用反相高效液相色谱法（RP-HPLC），使用迪马 Diamonsil C18（250 mm ×4．6 mm，5μm）色谱柱，以乙腈-0．2％氨水流动相，流速1．0 mL·min －1，柱温35℃，检测波长254 nm，进样量20μL。结果对叶百部碱线性范围为31．25～500．0 mg·L －1，r ＝0．9995，平均加样回收率（n ＝9）为99．88％，RSD 为0．26％。不同产地对叶百部根中对叶百部碱含量差异较大，其中河北对叶百部根中对叶百部碱含量较高为1514．80μg·g －1，广西产对叶百部碱含量较低为197．40μg·g －1。蜜炙后河北、广西产对叶百部根中对叶百部碱含量分别为919．60、129．76μg·g －1。结论该文建立的含量测定方法符合要求。对叶百部根经蜜炙后对叶百部碱的含量下降，表明蜜炙对叶百部根中生物碱类成分的含量产生了一定影响。
Objective To establish an HPLC method for the determination of tuberostemonine in root of Stemona tuberosa Lour.during stir-frying with honey.Methods HPLC and Diamonsil-C18 column （250 mm ×4.6 mm,5 μm）were adopted,with acetonitrile-0.2%ammonia as the mobile phase at the flow rate of 1.0 mL·min -1 .The column temperature was set at 35 ℃,and the UV detection wave-length was 254 nm.Sample quantity was 20 μL.Results Tuberostemonine showed a good linear relationship within the range of 31.25~500.0 mg·L -1 （r =0.999 5）,with the average recovery of 99.88% （n =9）,RSD being 0.26%.Tuberostemonine contents of Ste-mona tuberosa Lour.in different areas had great difference.The content of Stemona tuberosa Lour.purchased from Hebei was the high-est,1514.80 μg·g -1 ,and the lowest was purchased from Guangxi,197.40 μg·g -1 .After stir-frying with honey,the content of tu-berostemonine were decreased to 919.60,129.76 μg·g -1 ,respectively.Conclusions The method was simple and reliable,which met the requirements.The content of tuberostemonine is decreased by stir-frying with honey,indicating that honey-processed approach had an effect on the content of alkaloids in Stemona tuberosa Lour.
出处
《安徽医药》
CAS
2016年第8期1483-1486,共4页
Anhui Medical and Pharmaceutical Journal
基金
黑龙江省教育厅科学技术研究项目(12541925)
关键词
百部/化学
蜜炙
色谱法
高压液相
Stemona japonica/chemistry
Honey processing
Chromatography,high pressure liquid
