摘要
本研究以山竹不定胚诱导的无菌苗叶片为外植体,通过优化山竹叶片诱导再生的培养条件,建立了山竹再生培养技术体系方法。结果显示,叶片转入WPM+1.0 mg?L^(-1) GA3+1.0 mg?L^(-1) 6-BA+0.5 mg?L^(-1) TDZ培养基中,诱导结节性愈伤组织的比例为62.4%。转入添加0.7 mg?L^(-1) TDZ+0.5 mg?L^(-1) 6-BA的WPM胚性愈伤组织诱导培养基中,诱导出36%的球状胚性愈伤组织。8周后球状胚性愈伤组织转入不定芽诱导培养,在含有WPM+2.0 mg?L^(-1) 6-BA+0.5 mg?L^(-1) NAA的培养基中,增殖系数达12.54。当不定芽长到20 mm左右时,转接到生根培养基中进行生根诱导,诱导生根的最适培养基为1/2MS+0.5 mg?L^(-1)6-BA+0.5 mg?L^(-1) NAA+0.1 mg?L^(-1) IBA,生根率为85.2%。
This study established the system of in vitro culture and multiplication of mangosteen（Garcinia mangostana） using explants from germinated seedlings.The results showed that after 6 weeks of culture inoculation,high percentage（62.4%） of nodular callus formations were established from aseptically leaf explants on WPM medium with 1.0 mg·L-1 GA3＋1.0 mg·L-1 6-BA ＋0.5 mg·L-1 TDZ,and 36% globular callus formations were induced on WPM medium with 0.7 mg·L-1 TDZ＋0.5 mg·L-1 6-BA.After 8 weeks of culture,the multiplication coefficient of globular embryogenic callus reached 12.54 on WPM medium supplemented with 2.0 mg·L-16-BA and 0.5 mg·L-1 NAA.Subsequently,adventitious buds with 20 mm length were transferred to rooting medium for rooting culture.And the best medium for rooting was 1/2MS＋0.5 mg·L-1 6-BA＋0.5 mg·L-1 NAA＋0.1 mg·L-1 IBA＋1.0% AC,and the rooting rate was 85.2%.
出处
《植物生理学报》
CAS
CSCD
北大核心
2016年第9期1341-1346,共6页
Plant Physiology Journal
基金
海南省科技厅重点研发计划(ZDYF2016054)
中国热带农业科学院海口实验站科研专项经费(HKZKY140101)~~
关键词
山竹
结节愈伤组织
胚性愈伤组织
不定胚
Garcinia mangostana
nodular callus
embryonic callus
adventitious embryo
