摘要
目的 探究槲寄生滴眼液治疗去势雄兔干眼症的效果。方法 取健康成年雄兔48只,体质量1.5~2.0 kg,在无菌恒温条件下喂养。随机选取12只雄兔仅切开阴囊,不切除睾丸作为阴性对照组,眼部不给予任何处理。将其余36只雄兔行双侧睾丸切除术,切除辜丸及附辜,结扎输精管及精索静脉,制作去势雄兔模型。作去势雄兔模型后随机分成3组,仅行双侧睾丸切除术的阳性对照组、采用槲寄生滴眼液的槲寄生组、采用生理盐水滴眼液的生理盐水组。分别于治疗前及治疗后2周、4周、8周观察各组泪液分泌试验（SchirmerⅠtest,SⅠT）、角膜荧光素染色、泪液蛋白及角膜共聚焦显微镜下的情况。结果治疗4周、8周后,槲寄生组SⅠT较治疗前均有不同程度改善,差异均有统计学意义（均为P〈0.05）;而生理盐水组治疗2周、4周、8周后SⅠT较治疗前明显恶化,差异均有统计学意义（均为P〈0.05）;治疗2周、4周、8周后槲寄生组与生理盐水组各时间点SⅠT相比,差异均有统计学意义（均为P〈0.05）。治疗2周、4周、8周后,槲寄生组各时间点荧光素染色评分较治疗前均有不同程度改善,差异均有统计学意义（均为P〈0.05）,而生理盐水组荧光素染色评分较治疗前明显恶化,差异均有统计学意义（均为P〈0.05）;两组治疗后2周、4周、8周荧光素染色评分相比,差异均有统计学意义（均为P〈0.05）。槲寄生组与生理盐水组在治疗2周、4周、8周后泪液总蛋白量、乳铁蛋白、溶菌酶含量及淀粉酶活性相比,差异均有统计学意义（均为P〈0.05）。炎症细胞计数发现,经过8周治疗后,槲寄生组炎症细胞和上皮基底细胞密度分别为（75±12）mm-2和（2996±103）mm-2,而生理盐水组炎症细胞和上皮基底细胞密度分别为（234±26）mm-2和（4431±121）mm-2,两组比较,差异均有统计学意义（均为P〈0.05）。结论 槲寄生滴眼液可有�
Objective To investigate the experimental effects of mistletoe eye drops in prevention of xerophthalmia of castrated male rabbit. Methods Forty-eight healthy male rabbits were chosen, the body mass was 1.5 - 2.0 kg and fed under sterile constant temperature. The castrated dry eye model were established in 36 male rabbits, and randomly divided into three groups： only castrated group, mistletoe eye drops group,normal saline group. Other 12 rabbits were cut off the scrotum and served the negative control group. Schirmer＇ s test （ S [ T）, corneal fluorescein, tear proteins, and corneal confocal microscope were performed before treatment and 2 weeks, 1 month,2 months after treatment. Results There were significant differences in total protein, lactoferrin,lysozyme, amylase activity at 2 weeks, 4 weeks, 8 weeks after treatment of two groups （ all P 〈 0.05 ）. After 2 months of treatment, S IT, fluorescein staining scores were improved compared with before treatment in mistletoe eye drops group, the differ- ence was statistically significant （ all P 〈 0. 05 ）. And S l T, fluorescein staining scores were significantly worse at 2 weeks, 4 weeks, 8 weeks after treatment in saline eye drops group, the differences were statistically significant compared with before treat- ment （ all P 〈 0.05 ）. Tear total protein, lactoferrin, lysozyme and amylase activity at 2 weeks ,4 weeks ,8 weeks after treatment in two groups compared with before treatment had statistical differences （ all P 〈 0.05 ）. The density of inflammatory cells and epithelial basal cells respectively in the mistletoe eye drops group were （75 ± 12 ） mm 2 and （2996 ± 103 ）mm-2, while the density of inflammatory cells and epithelial basal cells in saline eye drops group respectively were（234 ± 26） mm 2 and（4431 ± 121 ） mm-2 ,there were statistical differences between the two groups （ all P 〈 0.05 ）. Conclusion Mistletoe eye drops can alleviate the signs and symptoms of dry eyes of castrated male rab- bits, whi
出处
《眼科新进展》
CAS
北大核心
2016年第10期915-918,922,共5页
Recent Advances in Ophthalmology
基金
国家自然科学基金资助(编号:81160118
81460092
81400372)
江西省远航工程(编号:2014022)
江西省青年科学基金(编号:20151BAB215016)
江西省科技支撑计划项目(编号:20151BBG70223)
江西省卫计委中医药科技计划项目(编号:2015JX126)~~
关键词
槲寄生滴眼液
去势雄兔
干眼症
mistletoe eye drops
castrated male rabbits
xerophthalmia
