摘要
目的评价过氧化物酶增殖体激活受体(peroxisome proliferator-activated receptor,PPAR)激动剂吡格列酮(pioglitazone,Pio)对癫痫持续状态大鼠海马神经元的保护作用及其可能的机制。方法选用健康成年雄性Wistar大鼠39只,随机分为三组:吡格列酮SE组(Pio组),生理盐水对照SE组(M组)及正常对照组(N组)(每组13只)。Pio组预先连续7天给予吡格列酮(10 mg/kg)灌胃处理,M组给予等剂量生理盐水(Na Cl)。其中Pio组及M组给予戊四氮(pentylenetetrazole,PTZ)40 mg/kg腹腔注射制备癫痫持续状态(status epilepticus,SE)大鼠模型。采用行为学观察癫痫持续状态大鼠的发作潜伏期及发作级别。Western blot检测癫痫大鼠海马组织中环氧化酶-2(cyclooxygenase,Cox-2)蛋白表达水平,ELISA法测定癫痫大鼠海马组织中前列腺素I2(prostaglandin I2,PGI2),前列腺素E2(prostaglandin E2,PGE2)的浓度。结果 Pio组较M组发作潜伏期延长(13.880±8.610)s vs(3.750±2.493)s,P<0.05,癫痫发作程度减轻。Pio组较M组Cox-2表达水平明显下降(P<0.05),Pio组及M组PGI2,PGE2浓度有所降低(0.724±0.025)pg/m L vs(0.734±0.027)pg/m L,P<0.05;(1.818±0.023)pg/m L vs(1.825±0.022)pg/m L,P<0.05。结论吡格列酮对于大鼠癫痫持续状态后神经元损伤具有保护作用;吡格列酮可能通过减轻炎症反应,进而起到保护神经元的作用。
Objective To evaluate the effects of peroxisome proliferator-activated receptor agonist pioglitazone on the hippocampus of status epilepticus rats and to explore its possible mechanism. Methods Thirty-nine healthy adult male Wistar rats were randomly divided into three groups：the SE model group treated with pioglitazone （Pio group）, the SE model group treated with saline （M group） and normal control group （N group）. Each group was treated with the method of gavage. The model treatment group rats were given pioglitazone solution （10 mg/kg） every day for 7 days before survived status epilepticus. M group was given equal volume saline. SE models were established by pentylenetetrazole intraperitoneal injection in Pio group and M group. The onset of SE models incubation period and the degree of seizure severity were detected by using behavioural observation. The expression of cox-2 in hippocampus of SE models was measured by Western blot, while the concentrations of PGI2 and PGE2 were detected by ELISA assay. Results The incubation period of Pio group was longer than that in M group, and the seizure severity was alleviated; the expression of Cox-2 in Pio group was significantly lower than that of M group ; while the concentrations of PGI2 and PGE2 in Pio group were lower than those of M group. Conclusion PPAR agonist, pioglitazone, has protective role against post-status epilepticus neuroton injury in rats; And the protective role of pioglitazone against post-status epilepticus neuron injury in rats may be ralated to its inflammatory reaction mechanism.
出处
《哈尔滨医科大学学报》
CAS
2016年第4期306-309,共4页
Journal of Harbin Medical University
