摘要
目的探讨秦皮甲素(esculin,ES)对D-半乳糖诱导大鼠晶状体损伤的作用及其机制。方法采用腹腔注射D-半乳糖建立白内障大鼠模型。雄性SD大鼠随机分为5组:正常对照组(control);D-半乳糖模型组(model);ES 30 mg·kg^(-1)给药组(ES30);ES 100 mg·kg^(-1)给药组(ES100);氨基胍100 mg·kg^(-1)给药组(AG)。给药结束后血糖检测仪检测大鼠空腹血糖;检测血清中糖化血清蛋白(GSP)含量;检测晶状体组织中丙二醛(MDA)含量、谷胱甘肽(GSH)含量、醛糖还原酶(AR)活性。结果与正常对照组相比,D-半乳糖诱导大鼠血糖显著增高(P<0.01),晶状体组织中MDA含量显著增高(P<0.05)、GSH水平显著降低(P<0.05)、AR活性显著增高(P<0.01)。与D-半乳糖诱导模型组比较,ES 30 mg·kg^(-1)组显著降低血清中GSP水平(P<0.05),显著降低晶状体MDA含量(P<0.05),显著增高晶状体GSH水平(P<0.05),显著降低晶状体AR活性(P<0.05)。结论秦皮甲素对D-半乳糖诱导晶状体损伤具有一定的改善作用,机制可能与其提高晶状体GSH含量、减少MDA产生、抑制AR活性相关。
OBJECTIVE To investigate the effect of esculin （ES） against lens injury induced by D-galactose in rats. METH- ODS Male SD rats were randomly divided into five groups： normal control group （control）, D-galactose model group （model）, ES 30 mg · kg-1 administered group （ES30）, ES 100 mg· kg-1 administered group （ES100） and aminoguanidine 100 mg ·kg-1 admin- istered group （AG）. After treatment, blood glucose and glycosylated serum protein （GSP） content in serum were measured. The con- tent of malondialdehyde （MDA）, the level of glutathione （GSH）, and the activity of aldose reductase （AR） in lens were also as- sayed. RESULTS Compared with control group, the level of blood glucose was significantly increased （P 〈 0.01 ） in D-galactose model rats. In addition, in the lens tissue of D-galactose-induced rats, the content of MDA was significantly decreased （P 〈 0. 05 ）, the level of GSH was significantly increased （P 〈 0.05 ）, and the activity of AR was significantly decreased （ P 〈 0.05 ）. However, co- treatment with ES 30 mg · kg- 1 significantly decreased the level of GSP in serum （P 〈 0. 05）, decreased the content of MDA （P 〈 0. 05）, increased the level of GSH （ P 〈 0.05 ）, and inhibited the activity of AR in lens （ P 〈 0. 05 ）. CONCLUSION Esculin could improve lens injury induced by D-galactose in rats. The mechanism may be related with increasing GSH level, decreasing MDA production, and inhibiting AR activity.
作者
杨欢
程笑
张惠芳
林溢煌
方莲花
王月华
杜冠华
YANG Huan CHENG Xiao ZHANG Hui-fang LIN Yi-huang FANG Lian-hua WANG Yue-hua DU Guan- hua(Beijing Key Laboratoty of Drug Target Identification and New Drug Screening, Institule of Materia Medica, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100050, China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2016年第19期1666-1670,共5页
Chinese Pharmaceutical Journal
基金
国家自然科学基金面上项目(81473383)
国家科技重大专项重大新药创制资助项目(2012ZX09103101-078,2013ZX09103001-008,2013ZX09102106)
