摘要
目的：观察罗哌卡因的急性毒性作用,并计算其致死剂量。方法：根据药理实验方法学改良寇氏（Karber）法,给大鼠尾静脉注射不同剂量罗哌卡因原液,观察并记录给药后大鼠的中毒表现及死亡情况;根据各组半数致死剂量（LD_（50））,计算半数致死量及95%可信区间（CI）。结果：罗哌卡因入血剂量在2.6 mg/kg时实验鼠可出现惊厥性收缩（抽搐）、翻正反射消失和自主活动减弱、呼吸抑制等,但无死亡;随着入血剂量增加,实验鼠可发生死亡,剂量增大至13.2 mg/kg,全部死亡。本研究资料测得致死剂量范围在2.6~12.6 mg/kg,其LD_（50）=7.29 mg/kg,95%CI（5.88,8.70）。解剖死亡大鼠,肉眼观察心、肝、脾、肺、肾及肠胃等主要脏器病理改变。结论：罗哌卡因小剂量入血后中枢神经系统中毒是可逆的,大剂量将引起不可逆的死亡。
Objective： To observe the acute toxic effect of Ropivacaine and its lethal dose. Method： According to the pharmacology experiment of improved Karber＇s method, poisoning manifestations and deaths of rats given different dose of ropivacaine were observed and recorded.LD50 and 95% confidence interval were calculated according to LD50 of different groups.Result： When inserted Ropivacaine with blood dose of 2.6 mg/kg, the rats showed symptoms of convulsive induced contraction （ convulsion ）, a loss of righting reflex, weakness of autonomic activities and respiratory depression without deaths.As the blood dose increased, the rats might die.When the blood dose reached 13.2 mg/kg, all the rats died.According to this research, lethal dose was between 2.6-12.6 mg/kg, whose LD50=7.29 mg/kg and 95%CI （ 5.88, 8.70 ） .After anatomy, the pathologic changes of the heart, liver, spleen, lung, kidney, intestines and stomach of the dead rats were given visual inspection.Conclusions： After a small dose of Ropivacaine into blood, the poisoning of central nervous system is reversible.A great dose of Ropivacaine causes reversible death.
出处
《中国医学创新》
CAS
2016年第27期17-19,共3页
Medical Innovation of China
基金
广东省深圳市卫生局科技计划项目(201202191)
关键词
局麻药
罗哌卡因
急性毒性
LD50
Local anesthetic
Ropivacaine
Acute toxicity
Lethal dose 50
