摘要
目的了解丙型肝炎病毒(HCV)在海南省汉族HCV慢性感染者中的基因型分布特点。方法选择在海南省人民医院就诊的176份汉族本土HCV慢性感染者(HCV-Ab和HCV RNA均阳性)血清标本,通过提取HCV-RNA,然后使用RT-PCR、巢式PCR(Nested-PCR)方法来扩增Core-E1区并测定其核苷酸序列,最后与已知HCV基因型序列构建系统发育树分析、确定其基因亚型及分布。结果成功提取HCV-RNA和测序的海南省汉族HCV慢性感染者标本为163例,检出阳性率为92.6%,其基因型分布和比率如下:1a型占3.1%(5/163),1b型占33.1%(54/163),2a型占6.7%(11/163),3a型占8.6%(14/163),3b型占14.7%(24/163),6a型占33.7%(55/163)。结论海南省汉族HCV慢性感染者中基因型分布与全国的HCV基因型分布一样,基因型为1a、1b、2a、3a、3b和6a。海南省汉族同属我国汉族HCV传播圈,本研究发现6a是海南省汉族HCV慢性感染者中最主要的基因亚型,提示6a可能在海南省汉族人群中发生迅速传播。
Objective To understand the distribution of hepatitis C virus（HCV）genotypes in ethnic Han of HainanProvince. Methods HCV- RNA（s）of 176 isolates of HCV- Ab and HCV- RNA positive serum samples were extracted,amplified in the HCV Core-E1 region by RT-PCR and Nested-PCR,and sequenced by using the phylogenetic tree to analyzeand determine the HCV genotype distribution of the ethnic Han from Hainan. Results HCV genotype distribution of 163（92.6%）isolates from ethnic Han in Hainan was successfully confirmed,and the genotype distribution and constituent ratioswere shown as followed： 1a was 3%（5/163）; 1b 33.1%（54/163）; 2a 6.7%（11/163）; 3a 8.6%（14/163）,3b 14.7%（24/163）,and 6a 33.7%（55/163）. Conclusions In comparison to the genotype of HCV in the rest of Chinese population,the subtypedistribution of chronic HCV infection persons of ethnic Han from Hainan Province,including 1a,1b,2a,3a,3b and 6a,are thesame. The ethnic Han in Hainan Province is a part of the Chinese mainland ethnic Han and they are in the same HCVtransmission circle. There is the highest percentage of subtype 6a in ethnic Han of Hainan Province means that the subtype 6ahas been spreading quickly in ethnic Han of Hainan Province.
作者
吴涛
王家健
许小珍
熊璐
王姣
王福利
林锋
WU Tao WANG Jia-jian XU Xiao-zhen XIONG Lu WANG Jiao WANG Fu-li LIN Feng(Department of lnfectious Disease ,Hainan General Hospital,Haikou,Hainan 570311, China)
出处
《中国热带医学》
CAS
2016年第9期873-876,共4页
China Tropical Medicine
基金
海南省应用技术研发与示范推广专项(No.ZDXM2015072)
关键词
丙型病毒性肝炎
基因型分布
海南省
汉族
Viral hepatitis C
Genotype distribution
Hainan Province
Ethnic Han
