摘要
目的:探讨小鼠肺癌细胞生长过程中自然杀伤性T细胞(NKT)和骨髓源性抑制细胞(MDSC)变化。方法:采用小鼠Lewis肺癌细胞系(LLC)制备小鼠皮下肿瘤模型,在肿瘤生长不同时间取鼠尾血,提取白细胞进行相关抗体染色,采用流式细胞仪进行NKT和MDSC细胞检测。结果:肺癌小鼠随着肿瘤增大外周血NKT细胞比例逐渐降低,而MDSC细胞比例逐渐增加,与正常对照小鼠相比,二者均存在显著差异。结论:小鼠NKT细胞和MDSC细胞在肺肿瘤发展过程中呈相反趋势变化,二者可作为肺癌发展的潜在监测指标。
Objective：To investigate the change of nature killer T cell（NKT） and myeloid derived suppressor cells（MDSC）during the development of mouse lung tumor.Methods：Lung tumor mouse models were made by subcutaneous injection of Lewis lung tumor cells（LLC）,peripheral blood leukocytes were extracted from mouse tail blood at different time points after LLC injection.NKT and MDSC were detected by flow cytometry after relative antibody staining.Results：With the increasing volume of lung tumor,the ratio of NKT cells decreased gradually,while the ratio of MDSC increased gradually in the peripheral blood of LLC-injected mice.Both NKT and MDSC showed significantly changes in LLC-injected mice compared with that of normal control mice.Conclusion：NKT and MDSC in LLC-injected mice show opposite changes during the development of lung tumor,so,they can be used as potential monitoring index for lung tumor development.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2016年第9期1272-1275,共4页
Chinese Journal of Immunology
基金
国家自然科学基金项目(81373111)
河北省自然科学基金项目(H2013209019)
华北理工大学大学生创新创业训练计划项目(X2015153)
关键词
肺癌
自然杀伤性T细胞
骨髓源性抑制细胞
Lung tumor
Nature killer T cells
Myeloid derived suppressor cells
