摘要
采用DGGE技术对南美白对虾体表与肠道的4个部位进行细菌菌群结构分析,结果表明:粪肠球菌和假单胞菌属是南美白对虾肠道细菌的主要成员;葡萄球菌属是体表的优势菌,尤其在腹部占据了绝对优势,包含3个菌种:表皮葡萄球菌、科氏葡萄球菌和巴氏葡萄球菌。不同部位的体表细菌存在差异,头胸部的葡萄球菌比腹部少,只有科氏葡萄球菌,而粪肠球菌与希瓦氏菌比腹部的多。头胸部有鳃,而腹部存在甲壳节与节的连接膜,这些生理结构的差异导致体表细菌菌群结构不同。DGGE图谱还揭示出一些常见的腐败菌分布是不同的,拟态弧菌和豚鼠气单胞菌只存在与肠道中,而希瓦氏菌属仅在体表被检测到。假单胞菌属的不同菌种在南美白对虾的体表与肠道有不同分布,DGGE甚至能检测到出现在体表与肠道的粪肠球菌的菌株不同。这些结果表明DGGE在菌株鉴定方面具有很高的灵敏性,有可能应用在腐败菌溯源方面。
DGGE（denaturing gradient gel electrophoresis） analysis was used to investigate the bacterial community of surface which is divided into cephalothorax and abdomen and gut flora of Penaeus vannamei. Both Enterococcus faecalis and Pseudomonas were the dominant bacteria in gut flora, while the genus of Staphylococcus, including 3 species： S.epidermidis, S. cohnii and S. pasteuri, was the primary member of the surface bacterial community of the shrimp, particularly in abdomen. Cephalothorax had less and weaker bands of Staphlococcus but stronger band of E. faecalis and Shewanella than abdomen. The difference of surface flora between cephalothorax and abdomen seemed to be related to their specific physiological structures, such as the gill in cephalothorax and seven carapace sections in abdomen. DGGE analysis also revealed the difference distribution on the common spoilage bacteria. Vibrio mimicus and Aeromonas caviae were only found in gut flora, and Shewanella was just displayed in surface bacterial community. And even the strain difference of E. faealis was detected between the same position bands in surface and in gut. These results suggested DGGE was sensitive in species identification and a potential method for spoilage bacteria retrospect.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2016年第8期218-224,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
杭州市重大科技创新项目(20112312A48)
浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地资助项目(2010DS700124-ZZ1604)
