摘要
目的:探讨INPP4B mRNA及蛋白在胃癌患者外周血中的表达水平,并分析其表达水平与临床病理参数(TNM分期、淋巴结转移和浸润深度)的关系。方法:应用实时荧光定量PCR(QRT-PCR)及酶联免疫吸附实验(ELISA)测定50例胃癌患者及30例健康志愿者外周血INPP4B mRNA及蛋白的表达水平。结果:胃癌患者外周血中INPP4B mRNA比健康志愿者外周血中INPP4B mRNA的表达水平显著下降(P<0.05),INPP4B蛋白在胃癌患者外周血中的浓度[(845.118±293.560)ng/L],显著低于健康志愿者外周血中的浓度[(2 089.204±603.829)ng/L](P<0.05)。INPP4B mRNA及蛋白的表达水平在胃癌外周血中与TNM分期、淋巴结转移和浸润深度相关(P<0.05)。结论:外周血中INPP4B mRNA及蛋白的表达与胃癌关系密切,可作为反映胃癌发生、发展过程的有效分子指标。
Objective To explore the mRNA and protein expression of INPP4B in peripheral blood of patients with gastric cancer. The relationship between the levels of INPP4B and the clinical characteristics, the TNM stage, lymph node metastasis and invasion depth was analyzed. Methods ：The mRNA and protein expression of INPP4B in peripheral blood were examined from 50 patients with gastric cancer and 30 cases of healthy volunteers by QRT - PCR and ELISA. Results： The mRNA expression of INPP4B in peripheral blood of gastric cancer patients was significantly de-creased compared to healthy volunteers（P 〈0. 05）. INPP4B concentration in peripheral blood of patients with gastric cancer[ （845. 118 ± 293. 560） ng/L] was significantly lower than that in peripheral blood of healthy volunteers [（2 089.204 ±603. 829 ）ng/L] （P 〈0. 05）. The mRNA and protein levels of INPP4B in peripheral blood of GC were related to TNM stage,lymph node metastasis,invasion depth（P 〈0.05）. Conclusion： The mRNA and proteinexpression of INPP4B in peripheral blood significantly correlated with gastric cancer progression, and can be as an ef-fective molecular marker for gastric cancer.
出处
《现代肿瘤医学》
CAS
2016年第21期3443-3446,共4页
Journal of Modern Oncology
关键词
胃癌
外周血
INPP4B
gastric cancer’peripheral blood, INPP4B
