摘要
2013年8月至2014年12月,从湖南湘西未接种任何疫苗(除猪瘟苗外)的山区小型猪场、农村传统饲养户以及部分野猪场采集了1 390份猪血清样品,通过酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA),血清学检测猪2型圆环病毒(Porcine circovirus type 2,PCV2)的血清抗体,对已确认为PCV2抗体阳性的血清进行猪蓝耳病毒(Porcine reproductive and respiratory syndrome virus,PRRSV),以及猪伪狂犬病毒(Pseudorabies virus,PRV)的血清抗体检测。检测结果显示PCV2血清抗体阳性率,小型猪场的为86%、农村传统饲养猪为78%、土猪场为69%、野猪场为25%。结果表明:湘西山区小型猪场、农村传统饲养户、部分野猪场的PCV2血清抗体阳性率不同,这种抗体阳性率的高低可能与饲养密度、饲养方式、品种和环境有关。本调查通过对湘西周边山区猪群的PCV2血清抗体阳性进行检测及统计分析,发现山区微小猪场及农户散养猪只具有较高的抗体阳性率,而抗体阳性率在一定程度上代表着PCV2的感染率。因此,加强PCV2免疫接种,将有利于防控PCV2相关性疾病的发生以及其他疾病继发的感染。
From August 2013 to December 2014, 1 390 serum samples were collected from non-any- vaccinated（ except swine fever） small-size swine farms in mountainous area, traditional swine farms in rural areas, and some of the wild boar field in Xiangxi of Hunan province, serum antibody of PCV2 were detected by enzyme-linked immuno sorbent assay （ELISA） testing. It has been recog- nized as PCV2 antibody positive sera that PPRSV and PRV were detected by ELISA. The results of PCV2 Serum antibody positive rate show that the positive rate of the small-size swine farms, the tra- ditional swine farms, the local swine, and the wild boar field were 86%, 78%, 69%, and 25%, respectively. These results showed that the positive rates of these swine farms in mountainous area, tra- ditional swine farms in rural areas, and some of the wild boar field were different, the infection rate of PCV2 is possibly related to breeding density, breeding tors. This investigation shows that the infection rates of the methods, varieties and environmental fac-small-size swine farms and the traditional swine farms are high by the testing and statistical analysis. Thus, porcine circovirus associated disease （PCVAD） and other diseases can be effectively control and prevent by strengthen vaccination with PCV2.
出处
《经济动物学报》
CAS
2016年第3期159-162,167,共5页
Journal of Economic Animal
基金
国家自科基金项目(31372406)
湖南省自科基金项目(2015JJ2082)
